Investigation of the mechanisms of RAMA on secretory protein trafficking in sporozoites, 2019.04 - 2022.03

State： Collaboration in Japan

Investigation of the mechanisms of RAMA on secretory protein trafficking in sporozoites, 2019.04 - 2022.03

State： Collaboration in Japan

Investigation of the mechanisms of RAMA on secretory protein trafficking in sporozoites, 2019.04 - 2022.03

State： Collaboration in Japan

Investigation of the mechanisms of RAMA on secretory protein trafficking in sporozoites, 2019.04 - 2022.03

Elucidation of the rule of Plasmodium parasite specific rhoptry protein in liver infection, 2018.04 - 2020.03

Summary： Rhoptry-associated leucine zipper-like protein 1 (RALP1) is one of rhoptry proteins which are conserved among in infective stage, merozoite and sporozoite. The attempts to generate RALP1 knockout parasites have failed possibly due to its essentiality for the parasite proliferation during blood stage. Therefore, until now, the function of RALP1 in sporozoites remains unelucidated.
To elucidate RALP1 function during sporozoite invasion, we applied the sporozoite stage-specific knockdown system by promoter swapping in the rodent malaria parasite strain, Plasmodium berghei. The amount of ralp1 mRNA in RALP1-cKD sporozoites were 10-fold lower than those in RALP1-control sporozoites. Parasitemias of RALP1-cKD sporozoite inoculated mice at 3 days after sporozoite inoculation were about 10-fold lower than those of RALP1-control sporozoite inoculated mice. It suggests that RALP1 plays an important role during liver infection stage of sporozoites.

Elucidation of the rule of Plasmodium parasite specific rhoptry protein in liver infection, 2018.04 - 2020.03

State： Individual
Summary： Rhoptry-associated leucine zipper-like protein 1 (RALP1) is one of rhoptry proteins which are conserved among in infective stage, merozoite and sporozoite. The attempts to generate RALP1 knockout parasites have failed possibly due to its essentiality for the parasite proliferation during blood stage. Therefore, until now, the function of RALP1 in sporozoites remains unelucidated.
To elucidate RALP1 function during sporozoite invasion, we applied the sporozoite stage-specific knockdown system by promoter swapping in the rodent malaria parasite strain, Plasmodium berghei. The amount of ralp1 mRNA in RALP1-cKD sporozoites were 10-fold lower than those in RALP1-control sporozoites. Parasitemias of RALP1-cKD sporozoite inoculated mice at 3 days after sporozoite inoculation were about 10-fold lower than those of RALP1-control sporozoite inoculated mice. It suggests that RALP1 plays an important role during liver infection stage of sporozoites.

Elucidation of the rule of Plasmodium parasite specific rhoptry protein in liver infection, 2018.04 - 2020.03

State： Individual
Summary： Rhoptry-associated leucine zipper-like protein 1 (RALP1) is one of rhoptry proteins which are conserved among in infective stage, merozoite and sporozoite. The attempts to generate RALP1 knockout parasites have failed possibly due to its essentiality for the parasite proliferation during blood stage. Therefore, until now, the function of RALP1 in sporozoites remains unelucidated.
To elucidate RALP1 function during sporozoite invasion, we applied the sporozoite stage-specific knockdown system by promoter swapping in the rodent malaria parasite strain, Plasmodium berghei. The amount of ralp1 mRNA in RALP1-cKD sporozoites were 10-fold lower than those in RALP1-control sporozoites. Parasitemias of RALP1-cKD sporozoite inoculated mice at 3 days after sporozoite inoculation were about 10-fold lower than those of RALP1-control sporozoite inoculated mice. It suggests that RALP1 plays an important role during liver infection stage of sporozoites.

Elucidation of the rule of Plasmodium parasite specific rhoptry protein in liver infection, 2018.04 - 2020.03

Summary： Rhoptry-associated leucine zipper-like protein 1 (RALP1) is one of rhoptry proteins which are conserved among in infective stage, merozoite and sporozoite. The attempts to generate RALP1 knockout parasites have failed possibly due to its essentiality for the parasite proliferation during blood stage. Therefore, until now, the function of RALP1 in sporozoites remains unelucidated.
To elucidate RALP1 function during sporozoite invasion, we applied the sporozoite stage-specific knockdown system by promoter swapping in the rodent malaria parasite strain, Plasmodium berghei. The amount of ralp1 mRNA in RALP1-cKD sporozoites were 10-fold lower than those in RALP1-control sporozoites. Parasitemias of RALP1-cKD sporozoite inoculated mice at 3 days after sporozoite inoculation were about 10-fold lower than those of RALP1-control sporozoite inoculated mice. It suggests that RALP1 plays an important role during liver infection stage of sporozoites.

Elucidation of the mechanisms of RON3 during sporozoites invasion of hepatocytes, 2016.04 - 2019.03

State： Collaboration in Japan
Summary： Malaria transmission to humans initiates by sporozoite infection of hepatocytes, which are inoculated by infected Anopheles mosquitoes. To develop the infection inhibitory vaccines, the step of sporozoite invasion of hepatocytes is one of the ideal targets, however its molecular mechanisms are not elucidated clearly. The aim of this study is to elucidate the molecular mechanisms of sporozoite invasion by focusing on RON3, the secretory protein stored in rhoptries in both merozoites and sporozoites. By generating sporozoite stage-specific RON3 conditional knockdown parasites in Plasmodium berghei, RON3 functions during sporozoite invasion was investigated in vitro and in vivo. Moreover, specific antibodies against partial recombinant RON3 were raised in rabbits to examine their inhibitory effects on sporozoite infection.

Elucidation of the mechanisms of RON3 during sporozoites invasion of hepatocytes, 2016.04 - 2019.03

State： Collaboration in Organization
Summary： Malaria transmission to humans initiates by sporozoite infection of hepatocytes, which are inoculated by infected Anopheles mosquitoes. To develop the infection inhibitory vaccines, the step of sporozoite invasion of hepatocytes is one of the ideal targets, however its molecular mechanisms are not elucidated clearly. The aim of this study is to elucidate the molecular mechanisms of sporozoite invasion by focusing on RON3, the secretory protein stored in rhoptries in both merozoites and sporozoites. By generating sporozoite stage-specific RON3 conditional knockdown parasites in Plasmodium berghei, RON3 functions during sporozoite invasion was investigated in vitro and in vivo. Moreover, specific antibodies against partial recombinant RON3 were raised in rabbits to examine their inhibitory effects on sporozoite infection.

Elucidation of the mechanisms of RON3 during sporozoites invasion of hepatocytes, 2016.04 - 2019.03

State： Collaboration in Organization
Summary： Malaria transmission to humans initiates by sporozoite infection of hepatocytes, which are inoculated by infected Anopheles mosquitoes. To develop the infection inhibitory vaccines, the step of sporozoite invasion of hepatocytes is one of the ideal targets, however its molecular mechanisms are not elucidated clearly. The aim of this study is to elucidate the molecular mechanisms of sporozoite invasion by focusing on RON3, the secretory protein stored in rhoptries in both merozoites and sporozoites. By generating sporozoite stage-specific RON3 conditional knockdown parasites in Plasmodium berghei, RON3 functions during sporozoite invasion was investigated in vitro and in vivo. Moreover, specific antibodies against partial recombinant RON3 were raised in rabbits to examine their inhibitory effects on sporozoite infection.

Elucidation of the mechanisms of RON3 during sporozoites invasion of hepatocytes, 2016.04 - 2019.03

Summary： Malaria transmission to humans initiates by sporozoite infection of hepatocytes, which are inoculated by infected Anopheles mosquitoes. To develop the infection inhibitory vaccines, the step of sporozoite invasion of hepatocytes is one of the ideal targets, however its molecular mechanisms are not elucidated clearly. The aim of this study is to elucidate the molecular mechanisms of sporozoite invasion by focusing on RON3, the secretory protein stored in rhoptries in both merozoites and sporozoites. By generating sporozoite stage-specific RON3 conditional knockdown parasites in Plasmodium berghei, RON3 functions during sporozoite invasion was investigated in vitro and in vivo. Moreover, specific antibodies against partial recombinant RON3 were raised in rabbits to examine their inhibitory effects on sporozoite infection.