Details of a Researcher

写真a

MIYATA Yuugo

Job title

Assistant Professor

Genome-wide screening for revealing molecular mechanisms of anhydrobiosis in Polypedilum vanderplanki, 2018.04 - 2021.03

Summary： Pv11 is a cultured cell line derived from embryos of the sleeping chironomid Polypedilum vanderplanki. Like the midge, Pv11 cells also display extreme desiccation tolerance; consequently, the cells can be stored in a dry state at room temperature. To apply the amazing ability to store biological things under dry conditions at room temperature, many researchers have tried to reveal the molecular mechanisms of the desiccation tolerance. However, only a few mechanisms were cleared, and most of them were still uncleared. So, I tried the genome-wide screening for understanding the molecular mechanisms of Pv11 cells.
At first, I constituted the experimental system for the screening. Through the construction, I identified several genes necessary for the anhydrobiotic ability of Pv11 cells.
Specific differentiation mechanisms of visceral adipogenic cells, 2015.04 - 2018.03

Summary： A large number of papers have shown that fat accumulation and the dysfunction of visceral adipose tissue cause abnormalities in whole body metabolic homeostasis. Recently, we developed a practical in vitro culture system for mesenteric adipocytes. Using the system, specific differentiation mechanisms of in vitro mesenteric adipogenic cells was shown.

Miyata Y, Segawa K. Protocol to analyze lipid asymmetry in the plasma membrane. STAR protocols. 2022.12; 3 (4): 101870. ( PubMed, DOI )
Miyata Yugo, Tokumoto Shoko, Arai Tomohiko, Shaikhutdinov Nurislam, Deviatiiarov Ruslan, Fuse Hiroto, Gogoleva Natalia, Garushyants Sofya, Cherkasov Alexander, Ryabova Alina, Gazizova Guzel, Cornette Richard, Shagimardanova Elena, Gusev Oleg, Kikawada Takahiro. Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11 GENES. 2022.03; 13 (3): ( PubMed, DOI )
High quality genome assembly of the anhydrobiotic midge provides insights on a single chromosome-based emergence of extreme desiccation tolerance 2022.03; 4 (2): ( DOI )
Cas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells 2021.12; 11 (1): ( DOI )
Genome-Wide Role of HSF1 in Transcriptional Regulation of Desiccation Tolerance in the Anhydrobiotic Cell Line, Pv11. 2021.05; 22 (11): ( PubMed, DOI )
Low melting point agarose (LMPA)-immobilization method to improve functional assay of olfactory receptors in Sf9 cells 2020; ( DOI )
The Physiological Functions of a Model Peptide of G3LEA in Living Cells 2019; ( DOI )
Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11 2017; 21 (1): 65-72. ( PubMed, DOI )