基本情報

写真a

藤吉 好則(フジヨシ ヨシノリ)

FUJIYOSHI Yoshinori


職名

高等研究院特別栄誉教授

ホームページ

http://www.tmd.ac.jp/cesp/index.html

研究分野・キーワード

チャネル、単粒子解析、膜タンパク質、構造生理学、クライオ電子顕微鏡学

出身学校 【 表示 / 非表示

  • 名古屋大学  理学部  化学科  1971年03月  卒業

出身大学院 【 表示 / 非表示

  • 京都大学  理学研究科  博士課程  1979年03月  単位取得満期退学

取得学位 【 表示 / 非表示

  • 理学博士  京都大学

経歴(学内) 【 表示 / 非表示

  • 2019年04月
    -
    2022年06月
    東京医科歯科大学 高等研究院 卓越研究部門 高等研究院特別栄誉教授
  • 2022年07月
    -
    現在
    東京医科歯科大学 高等研究院 卓越研究部門 細胞構造生理学研究室 高等研究院特別栄誉教授

所属学協会 【 表示 / 非表示

  • 日本生物物理学会

  • 日本顕微鏡学会

  • 日本生化学会

  • 日本分子生物学会

研究分野 【 表示 / 非表示

  • 構造生物化学

  • 細胞生物学

  • 分子生物学

  • 生物物理学

  • 薬系化学、創薬科学

  • 医療薬学

  • 生理学

▼全件表示

 

競争的資金等の研究課題 【 表示 / 非表示

  • ユビキチン化によるアクアポリン2の輸送制御機構の研究

    文部科学省/日本学術振興会 : 2021年 - 2022年

  • チャネルを中心とした構造生理学的研究

    文部科学省/日本学術振興会

  • クライオ電子顕微鏡法の開発によるチャネルの構造生理学研究

    文部科学省/日本学術振興会

論文・総説 【 表示 / 非表示

  1. Yukihiro Tanimura, Yoko Hiroaki, Masahiro Mori, Yoshinori Fujiyoshi. Cell-based flow cytometry assay for simultaneous detection of multiple autoantibodies in a single serum sample. Anal Biochem. 2022.08; 650 114721. ( PubMed, DOI )

  2. Katerina Naydenova, Akiko Kamegawa, Mathew J Peet, Richard Henderson, Yoshinori Fujiyoshi, Christopher J Russo. On the reduction in the effects of radiation damage to two-dimensional crystals of organic and biological molecules at liquid-helium temperature. Ultramicroscopy. 2022.07; 237 113512. ( PubMed, DOI )

  3. Maki Kuzuya, Hidemi Hirano, Kenichi Hayashida, Masakatsu Watanabe, Kazumi Kobayashi, Tohru Terada, Md Iqbal Mahmood, Florence Tama, Kazutoshi Tani, Yoshinori Fujiyoshi, Atsunori Oshima. Structures of human pannexin-1 in nanodiscs reveal gating mediated by dynamic movement of the N terminus and phospholipids. Sci Signal. 2022.02; 15 (720): eabg6941. ( PubMed, DOI )

  4. Yukako Oda, Chisato Takahashi, Shota Harada, Shun Nakamura, Daxiao Sun, Kazumi Kiso, Yuko Urata, Hitoshi Miyachi, Yoshinori Fujiyoshi, Alf Honigmann, Seiichi Uchida, Yasushi Ishihama, Fumiko Toyoshima. Discovery of anti-inflammatory physiological peptides that promote tissue repair by reinforcing epithelial barrier formation. Sci Adv. 2021.11; 7 (47): eabj6895. ( PubMed, DOI )

  5. Hanayo Nakanishi, Tomohiro Nishizawa, Katsumori Segawa, Osamu Nureki, Yoshinori Fujiyoshi, Shigekazu Nagata, Kazuhiro Abe. Transport Cycle of Plasma Membrane Flippase ATP11C by Cryo-EM. Cell Rep. 2020.09; 32 (13): 108208. ( PubMed, DOI )

  6. Shimada Atsuhiro, Kozai Daisuke, Nishikawa Kouki, Fujiyoshi Yoshinori, Sho Gyokucho, Mizutani Takumi, Tani Kazutoshi. クライオ電子顕微鏡を用いたシトクロム酸化酵素とシトクロムcの複合体構造解析(The structural analysis of cytochrome c oxidase complexed with cytochrome c using cryo-electron microscopy) 生物物理. 2020.08; 60 (Suppl.1-2): S158. ( 医中誌 )

  7. Irie Katsumasa, Shimomura Takushi, Yonekawa Yoshiki, Nagura Hitoshi, Tateyama Michihiro, Fujiyoshi Yoshinori. 天然の原核生物由来カルシウムチャネルにおけるイオン透過選択性の進化とその決定残基の同定(The selectivity determinant and evolution of a native prokaryotic voltage-dependent calcium channel) 生物物理. 2020.08; 60 (Suppl.1-2): S248. ( 医中誌 )

  8. Hanayo Nakanishi, Katsumasa Irie, Katsumori Segawa, Kazuya Hasegawa, Yoshinori Fujiyoshi, Shigekazu Nagata, Kazuhiro Abe. Crystal structure of a human plasma membrane phospholipid flippase. J Biol Chem. 2020.07; 295 (30): 10180-10194. ( PubMed, DOI )

  9. B. Burendei, R. Shinozaki, M. Watanabe, T. Terada, K. Tani, Y. Fujiyoshi and A. Oshima. Cryo-EM structures of undocked innexin-6 hemichannels in phospholipids. Sci. Adv.. 2020.02; 6 (7): ( DOI )

  10. Takushi Shimomura, Yoshiki Yonekawa, Hitoshi Nagura, Michihiro Tateyama, Yoshinori Fujiyoshi, Katsumasa Irie. A native prokaryotic voltage-dependent calcium channel with a novel selectivity filter sequence. Elife. 2020.02; 9 ( PubMed, DOI )

  11. K. Yamamoto, V. Dubey, K. Irie, H. Nakanishi, H. Khandelia, Y. Fujiyoshi and K. Abe. A single K+-binding site in the crystal structure of the gastric proton pump. eLife. 2019.08; 8 ( DOI )

  12. Shun Nakamura, Katsumasa Irie, Hiroo Tanaka, Kouki Nishikawa, Hiroshi Suzuki, Yasunori Saitoh, Atsushi Tamura, Sachiko Tsukita & Yoshinori Fujiyoshi. Morphologic determinant of tight junctions revealed by claudin-3 structures Nature Communications. 2019.02; 10 816. ( DOI )

  13. Hiroki Yamaguchi, Akiko Kamegawa, Kunio Nakata, Tatsuki Kashiwagi, Toshimi Mizukoshi, Yoshinori Fujiyoshi, Kazutoshi Tani. Structural insights into thermostabilization of leucine dehydrogenase from its atomic structure by cryo-electron microscopy Journal of Structural Biology. 2019.01; 205 (1): 11-21. ( DOI )

  14. 藤吉 好則. クライオ電子顕微鏡が激変させたタンパク質の立体構造解析技術 日本臨床免疫学会総会プログラム・抄録集. 2020.10; 48回 84. ( 医中誌 )

  15. 中西 華代, 入江 克雅, 瀬川 勝盛, 長田 重一, 長谷川 和也, 藤吉 好則, 阿部 一啓. ヒト細胞膜フリッパーゼATP11CのX線結晶構造解析 日本生化学会大会プログラム・講演要旨集. 2020.09; 93回 [P-132]. ( 医中誌 )

  16. 山口 浩輝, 亀川 亜希子, 中田 國夫, 柏木 立己, 高橋 一敏, 田上 宇乃, 巽 萌美, 水越 利巳, 宮野 博, 藤吉 好則, 谷 一寿. 酵素には世界と未来を変える力がある-今こそ酵素を見つめ直す2020- クライオ電子顕微鏡によるロイシン脱水素酵素の耐熱化機構解明 日本生化学会大会プログラム・講演要旨集. 2020.09; 93回 [3S08m-04]. ( 医中誌 )

  17. Abe K, Irie K, Nakanishi H, Suzuki H, Fujiyoshi Y. Crystal structures of the gastric proton pump. Nature. 2018.04; 556 (7700): 214-218. ( PubMed, DOI )

  18. Nakamura S, Fujiyoshi Y, Irie K. Enhancement of the thermostability of mouse claudin-3 on complex formation with the carboxyl-terminal region of Clostridium perfringens enterotoxin improves crystal quality. Acta crystallographica. Section F, Structural biology communications. 2018.03; 74 (Pt 3): 150-155. ( PubMed, DOI )

  19. Irie K, Haga Y, Shimomura T, Fujiyoshi Y. Optimized expression and purification of NavAb provide the structural insight into the voltage dependence. FEBS letters. 2018.01; 592 (2): 274-283. ( PubMed, DOI )

  20. Shihoya W, Nishizawa T, Yamashita K, Inoue A, Hirata K, Kadji FMN, Okuta A, Tani K, Aoki J, Fujiyoshi Y, Doi T, Nureki O. X-ray structures of endothelin ET<sub>B</sub> receptor bound to clinical antagonist bosentan and its analog. Nature structural & molecular biology. 2017.09; 24 (9): 758-764. ( PubMed, DOI )

  21. Abe K, Shimokawa J, Naito M, Munson K, Vagin O, Sachs G, Suzuki H, Tani K, Fujiyoshi Y. The cryo-EM structure of gastric H<sup>+</sup>,K<sup>+</sup>-ATPase with bound BYK99, a high-affinity member of K<sup>+</sup>-competitive, imidazo[1,2-a]pyridine inhibitors. Scientific reports. 2017.07; 7 (1): 6632. ( PubMed, DOI )

  22. Suzuki H, Tani K, Fujiyoshi Y. Crystal structures of claudins: insights into their intermolecular interactions. Annals of the New York Academy of Sciences. 2017.06; 1397 (1): 25-34. ( PubMed, DOI )

  23. Oshima A, Tani K, Fujiyoshi Y. Atomic structure of the innexin-6 gap junction channel determined by cryo-EM. Nature communications. 2016.12; 7 13681. ( PubMed, DOI )

  24. Shihoya W, Nishizawa T, Okuta A, Tani K, Dohmae N, Fujiyoshi Y, Nureki O, Doi T. Activation mechanism of endothelin ET<sub>B</sub> receptor by endothelin-1. Nature. 2016.09; 537 (7620): 363-368. ( PubMed, DOI )

  25. Shimomura T, Irie K, Fujiyoshi Y. Molecular determinants of prokaryotic voltage-gated sodium channels for recognition of local anesthetics. The FEBS journal. 2016.08; 283 (15): 2881-2895. ( PubMed, DOI )

  26. Abe K, Fujiyoshi Y. Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer. Current opinion in structural biology. 2016.08; 39 71-78. ( PubMed, DOI )

  27. Okuta A, Tani K, Nishimura S, Fujiyoshi Y, Doi T. Thermostabilization of the Human Endothelin Type B Receptor. Journal of molecular biology. 2016.06; 428 (11): 2265-2274. ( PubMed, DOI )

  28. Kamegawa A, Hiroaki Y, Tani K, Fujiyoshi Y. Two-dimensional crystal structure of aquaporin-4 bound to the inhibitor acetazolamide. Microscopy (Oxford, England). 2016.04; 65 (2): 177-184. ( PubMed, DOI )

  29. Nagura H, Doi T, Fujiyoshi Y. Characterization of physiological phenotypes of dentate gyrus synapses of PDZ1/2 domain-deficient PSD-95-knockin mice. The European journal of neuroscience. 2016.03; 43 (5): 618-625. ( PubMed, DOI )

  30. Oshima A, Matsuzawa T, Murata K, Tani K, Fujiyoshi Y. Hexadecameric structure of an invertebrate gap junction channel. Journal of molecular biology. 2016.03; 428 (6): 1227-1236. ( PubMed, DOI )

  31. Bando Y, Irie K, Shimomura T, Umeshima H, Kushida Y, Kengaku M, Fujiyoshi Y, Hirano T, Tagawa Y. Control of Spontaneous Ca2+ Transients Is Critical for Neuronal Maturation in the Developing Neocortex. Cerebral cortex (New York, N.Y. : 1991). 2016.01; 26 (1): 106-117. ( PubMed, DOI )

  32. Tanaka H, Yamamoto Y, Kashihara H, Yamazaki Y, Tani K, Fujiyoshi Y, Mineta K, Takeuchi K, Tamura A, Tsukita S. Claudin-21 Has a Paracellular Channel Role at Tight Junctions. Molecular and cellular biology. 2016.01; 36 (6): 954-964. ( PubMed, DOI )

  33. Hauer F, Gerle C, Fischer N, Oshima A, Shinzawa-Itoh K, Shimada S, Yokoyama K, Fujiyoshi Y, Stark H. GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM. Structure (London, England : 1993). 2015.09; 23 (9): 1769-1775. ( PubMed, DOI )

  34. Minatohara K, Murata Y, Fujiyoshi Y, Doi T. An intracellular domain with a novel sequence regulates cell surface expression and synaptic clustering of leucine-rich repeat transmembrane proteins in hippocampal neurons. Journal of neurochemistry. 2015.08; 134 (4): 618-628. ( PubMed, DOI )

  35. Jiko C, Davies KM, Shinzawa-Itoh K, Tani K, Maeda S, Mills DJ, Tsukihara T, Fujiyoshi Y, Kühlbrandt W, Gerle C. Bovine F1Fo ATP synthase monomers bend the lipid bilayer in 2D membrane crystals. eLife. 2015.03; 4 e06119. ( PubMed, DOI )

  36. Saitoh Y, Suzuki H, Tani K, Nishikawa K, Irie K, Ogura Y, Tamura A, Tsukita S, Fujiyoshi Y. Tight junctions. Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin. Science (New York, N.Y.). 2015.02; 347 (6223): 775-778. ( PubMed, DOI )

  37. Suzuki H, Tani K, Tamura A, Tsukita S, Fujiyoshi Y. Model for the architecture of claudin-based paracellular ion channels through tight junctions. Journal of molecular biology. 2015.01; 427 (2): 291-297. ( PubMed, DOI )

  38. Fujiyoshi Y. Development of the field of structural physiology. Proceedings of the Japan Academy. Series B, Physical and biological sciences. 2015; 91 (9): 447-468. ( PubMed, DOI )

  39. Abe K, Tani K, Fujiyoshi Y. Systematic comparison of molecular conformations of H+,K+-ATPase reveals an important contribution of the A-M2 linker for the luminal gating. The Journal of biological chemistry. 2014.10; 289 (44): 30590-30601. ( PubMed, DOI )

  40. Tani K, Fujiyoshi Y. Water channel structures analysed by electron crystallography. Biochimica et biophysica acta. 2014.05; 1840 (5): 1605-1613. ( PubMed, DOI )

  41. Suzuki H, Nishizawa T, Tani K, Yamazaki Y, Tamura A, Ishitani R, Dohmae N, Tsukita S, Nureki O, Fujiyoshi Y. Crystal structure of a claudin provides insight into the architecture of tight junctions. Science (New York, N.Y.). 2014.04; 344 (6181): 304-307. ( PubMed, DOI )

  42. Morito D, Nishikawa K, Hoseki J, Kitamura A, Kotani Y, Kiso K, Kinjo M, Fujiyoshi Y, Nagata K. Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state. Scientific reports. 2014.03; 4 4442. ( PubMed, DOI )

  43. Yang F, Abe K, Tani K, Fujiyoshi Y. Carbon sandwich preparation preserves quality of two-dimensional crystals for cryo-electron microscopy. Microscopy (Oxford, England). 2013.12; 62 (6): 597-606. ( PubMed, DOI )

  44. Tsai CJ, Tani K, Irie K, Hiroaki Y, Shimomura T, McMillan DG, Cook GM, Schertler GF, Fujiyoshi Y, Li XD. Two alternative conformations of a voltage-gated sodium channel. Journal of molecular biology. 2013.11; 425 (22): 4074-4088. ( PubMed, DOI )

  45. Tani K, Arthur CP, Tamakoshi M, Yokoyama K, Mitsuoka K, Fujiyoshi Y, Gerle C. Visualization of two distinct states of disassembly in the bacterial V-ATPase from Thermus thermophilus. Microscopy (Oxford, England). 2013.08; 62 (4): 467-474. ( PubMed, DOI )

  46. Fujiyoshi Y, Tanji T, Osakabe N, Hirayama T. Special Issue: "Tribute to Akira Tonomura". Foreword. Microscopy (Oxford, England). 2013.06; 62 Suppl 1 S1. ( PubMed, DOI )

  47. Oshima A, Matsuzawa T, Nishikawa K, Fujiyoshi Y. Oligomeric structure and functional characterization of Caenorhabditis elegans Innexin-6 gap junction protein. The Journal of biological chemistry. 2013.04; 288 (15): 10513-10521. ( PubMed, DOI )

  48. Mori H, Fujiyoshi Y. Preface on the occasion of the inauguration issue of Microscopy. Microscopy (Oxford, England). 2013.02; 62 (1): 1-2. ( PubMed, DOI )

  49. Goda M, Fujiyoshi Y, Sugimoto M, Fujii R. Novel dichromatic chromatophores in the integument of the mandarin fish Synchiropus splendidus. The Biological bulletin. 2013.02; 224 (1): 14-17. ( PubMed, DOI )

  50. Minatohara K, Ichikawa SH, Seki T, Fujiyoshi Y, Doi T. Ligand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95. Neuroscience letters. 2013.01; 533 44-49. ( PubMed, DOI )

  51. Fujiyoshi Y. Low dose techniques and cryo-electron microscopy. Methods in molecular biology (Clifton, N.J.). 2013; 955 103-118. ( PubMed, DOI )

  52. Suzuki H, Ito Y, Yamazaki Y, Mineta K, Uji M, Abe K, Tani K, Fujiyoshi Y, Tsukita S. The four-transmembrane protein IP39 of Euglena forms strands by a trimeric unit repeat. Nature communications. 2013; 4 1766. ( PubMed, DOI )

  53. Fujiyoshi Y. Future directions of electron crystallography. Methods in molecular biology (Clifton, N.J.). 2013; 955 551-568. ( PubMed, DOI )

  54. Nagura H, Ishikawa Y, Kobayashi K, Takao K, Tanaka T, Nishikawa K, Tamura H, Shiosaka S, Suzuki H, Miyakawa T, Fujiyoshi Y, Doi T. Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice. Molecular brain. 2012.12; 5 43. ( PubMed, DOI )

  55. Abe K, Tani K, Friedrich T, Fujiyoshi Y. Cryo-EM structure of gastric H+,K+-ATPase with a single occupied cation-binding site. Proceedings of the National Academy of Sciences of the United States of America. 2012.11; 109 (45): 18401-18406. ( PubMed, DOI )

  56. Unwin N, Fujiyoshi Y. Gating movement of acetylcholine receptor caught by plunge-freezing. Journal of molecular biology. 2012.10; 422 (5): 617-634. ( PubMed, DOI )

  57. Irie K, Shimomura T, Fujiyoshi Y. The C-terminal helical bundle of the tetrameric prokaryotic sodium channel accelerates the inactivation rate. Nature communications. 2012.04; 3 793. ( PubMed, DOI )

  58. Rico F, Oshima A, Hinterdorfer P, Fujiyoshi Y, Scheuring S. Two-dimensional kinetics of inter-connexin interactions from single-molecule force spectroscopy. Journal of molecular biology. 2011.09; 412 (1): 72-79. ( PubMed, DOI )

  59. Goda M, Ohata M, Ikoma H, Fujiyoshi Y, Sugimoto M, Fujii R. Integumental reddish-violet coloration owing to novel dichromatic chromatophores in the teleost fish, Pseudochromis diadema. Pigment cell & melanoma research. 2011.08; 24 (4): 614-617. ( PubMed, DOI )

  60. Ohta Y, Nishikawa K, Hiroaki Y, Fujiyoshi Y. Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish. Journal of structural biology. 2011.07; 175 (1): 49-61. ( PubMed, DOI )

  61. Wakao S, Kitada M, Kuroda Y, Shigemoto T, Matsuse D, Akashi H, Tanimura Y, Tsuchiyama K, Kikuchi T, Goda M, Nakahata T, Fujiyoshi Y, Dezawa M. Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proceedings of the National Academy of Sciences of the United States of America. 2011.06; 108 (24): 9875-9880. ( PubMed, DOI )

  62. Yakata K, Tani K, Fujiyoshi Y. Water permeability and characterization of aquaporin-11. Journal of structural biology. 2011.05; 174 (2): 315-320. ( PubMed, DOI )

  63. Fujiyoshi Y. Structural physiology based on electron crystallography. Protein science : a publication of the Protein Society. 2011.05; 20 (5): 806-817. ( PubMed, DOI )

  64. Shimomura T, Irie K, Nagura H, Imai T, Fujiyoshi Y. Arrangement and mobility of the voltage sensor domain in prokaryotic voltage-gated sodium channels. The Journal of biological chemistry. 2011.03; 286 (9): 7409-7417. ( PubMed, DOI )

  65. Oshima A, Tani K, Toloue MM, Hiroaki Y, Smock A, Inukai S, Cone A, Nicholson BJ, Sosinsky GE, Fujiyoshi Y. Asymmetric configurations and N-terminal rearrangements in connexin26 gap junction channels. Journal of molecular biology. 2011.01; 405 (3): 724-735. ( PubMed, DOI )

  66. Abe K, Tani K, Fujiyoshi Y. Conformational rearrangement of gastric H(+),K(+)-ATPase induced by an acid suppressant. Nature communications. 2011.01; 2 155. ( PubMed, DOI )

  67. Fujiyoshi Y. Electron crystallography for structural and functional studies of membrane proteins. Journal of electron microscopy. 2011; 60 Suppl 1 S149-59. ( PubMed, DOI )

  68. Mitsuma T, Tani K, Hiroaki Y, Kamegawa A, Suzuki H, Hibino H, Kurachi Y, Fujiyoshi Y. Influence of the cytoplasmic domains of aquaporin-4 on water conduction and array formation. Journal of molecular biology. 2010.10; 402 (4): 669-681. ( PubMed, DOI )

  69. Matsuse D, Kitada M, Kohama M, Nishikawa K, Makinoshima H, Wakao S, Fujiyoshi Y, Heike T, Nakahata T, Akutsu H, Umezawa A, Harigae H, Kira J, Dezawa M. Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration. Journal of neuropathology and experimental neurology. 2010.09; 69 (9): 973-985. ( PubMed, DOI )

  70. Nagura H, Irie K, Imai T, Shimomura T, Hige T, Fujiyoshi Y. Evidence for lateral mobility of voltage sensors in prokaryotic voltage-gated sodium channels. Biochemical and biophysical research communications. 2010.08; 399 (3): 341-346. ( PubMed, DOI )

  71. Kuroda Y, Kitada M, Wakao S, Nishikawa K, Tanimura Y, Makinoshima H, Goda M, Akashi H, Inutsuka A, Niwa A, Shigemoto T, Nabeshima Y, Nakahata T, Nabeshima Y, Fujiyoshi Y, Dezawa M. Unique multipotent cells in adult human mesenchymal cell populations. Proceedings of the National Academy of Sciences of the United States of America. 2010.05; 107 (19): 8639-8643. ( PubMed, DOI )

  72. Abe K, Tani K, Fujiyoshi Y. Structural and functional characterization of H+, K+-ATPase with bound fluorinated phosphate analogs. Journal of structural biology. 2010.04; 170 (1): 60-68. ( PubMed, DOI )

  73. Irie K, Kitagawa K, Nagura H, Imai T, Shimomura T, Fujiyoshi Y. Comparative study of the gating motif and C-type inactivation in prokaryotic voltage-gated sodium channels. The Journal of biological chemistry. 2010.02; 285 (6): 3685-3694. ( PubMed, DOI )

  74. Abe K, Tani K, Nishizawa T, Fujiyoshi Y. [Novel ratchet mechanism of gastric H(+), K(+)-ATPase revealed by electron crystallography of two-dimensional crystals]. Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan. 2010.02; 130 (2): 205-210. ( PubMed, DOI )

  75. Schenk AD, Hite RK, Engel A, Fujiyoshi Y, Walz T. Electron crystallography and aquaporins. Methods in enzymology. 2010; 483 91-119. ( PubMed, DOI )

  76. Inutsuka A, Goda M, Fujiyoshi Y. Calyculin A-induced neurite retraction is critically dependent on actomyosin activation but not on polymerization state of microtubules. Biochemical and biophysical research communications. 2009.12; 390 (4): 1160-1166. ( PubMed, DOI )

  77. Noma K, Kimura K, Minatohara K, Nakashima H, Nagao Y, Mizoguchi A, Fujiyoshi Y. Triple N-glycosylation in the long S5-P loop regulates the activation and trafficking of the Kv12.2 potassium channel. The Journal of biological chemistry. 2009.11; 284 (48): 33139-33150. ( PubMed, DOI )

  78. Kuwahara Y, Unzai S, Nagata T, Hiroaki Y, Yokoyama H, Matsui I, Ikegami T, Fujiyoshi Y, Hiroaki H. Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii. Biophysical journal. 2009.10; 97 (7): 2034-2043. ( PubMed, DOI )

  79. Fujiyoshi Y. [Observation of molecular structure of membrane proteins]. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 2009.08; 54 (10): 1300-1306. ( PubMed )

  80. Abe K, Tani K, Nishizawa T, Fujiyoshi Y. Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle. The EMBO journal. 2009.06; 28 (11): 1637-1643. ( PubMed, DOI )

  81. Tani K, Mitsuma T, Hiroaki Y, Kamegawa A, Nishikawa K, Tanimura Y, Fujiyoshi Y. Mechanism of aquaporin-4's fast and highly selective water conduction and proton exclusion. Journal of molecular biology. 2009.06; 389 (4): 694-706. ( PubMed, DOI )

  82. Tanimura Y, Hiroaki Y, Fujiyoshi Y. Acetazolamide reversibly inhibits water conduction by aquaporin-4. Journal of structural biology. 2009.04; 166 (1): 16-21. ( PubMed, DOI )

  83. Maeda S, Nakagawa S, Suga M, Yamashita E, Oshima A, Fujiyoshi Y, Tsukihara T. Structure of the connexin 26 gap junction channel at 3.5 A resolution. Nature. 2009.04; 458 (7238): 597-602. ( PubMed, DOI )

  84. Mio K, Ogura T, Yamamoto T, Hiroaki Y, Fujiyoshi Y, Kubo Y, Sato C. Reconstruction of the P2X(2) receptor reveals a vase-shaped structure with lateral tunnels above the membrane. Structure (London, England : 1993). 2009.02; 17 (2): 266-275. ( PubMed, DOI )

  85. Walz T, Fujiyoshi Y, Engel A. The AQP structure and functional implications. Handbook of experimental pharmacology. 2009; (190): 31-56. ( PubMed, DOI )

  86. Tani K, Hiroaki Y, Fujiyoshi Y. [Aquaporin-4]. Rinsho shinkeigaku = Clinical neurology. 2008.11; 48 (11): 941-944. ( PubMed )

  87. Fujiyoshi Y, Unwin N. Electron crystallography of proteins in membranes. Current opinion in structural biology. 2008.10; 18 (5): 587-592. ( PubMed, DOI )

  88. Sakisaka T, Yamamoto Y, Mochida S, Nakamura M, Nishikawa K, Ishizaki H, Okamoto-Tanaka M, Miyoshi J, Fujiyoshi Y, Manabe T, Takai Y. Dual inhibition of SNARE complex formation by tomosyn ensures controlled neurotransmitter release. The Journal of cell biology. 2008.10; 183 (2): 323-337. ( PubMed, DOI )

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  1. 藤吉好則. Cryo-EM changes structural biology [Tutorial Lecture]. ALC '19 2019.10.23

  2. 藤吉好則. Structure-guided drug development by cryo-EM [Plenary Lecture]. International Symposium on Diffraction Structural Biology 2019 2019.10.18

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  17. 藤吉好則. High resolution structure analyses by cryo-EM. Scottish Centre for Macromolecular Imaging Opening Symposium 2018.09.10 Glasgow (UK)

  18. 藤吉好則. 構造生物学を激変させたクライオ電顕. 第34回 分析電子顕微鏡討論会 2018.09.05 幕張

  19. 藤吉好則. 構造生理学と創薬. 第58回生物物理若手の会 夏の学校 2018.08.28 岐阜

  20. 藤吉好則. Drug Rescuing by Cryo-EM. 第18回国際薬理学・臨床薬理学会議 2018.07 京都

  21. 藤吉好則. セッション4 クライオ電顕の現状と展望. AMO討論会 2018.06.16 仙台

  22. 藤吉好則. クライオ電顕法の発展と創薬応用への可能性. 有機合成化学協会 2018.01.12 東京

  23. 藤吉好則. Drug Rescuing based on structure analyses by Cryo-EM. 日本薬物動態学会第33回年会/MDO国際合同学会 2018.01.03 金沢

  24. 藤吉好則. Drug Rescuing Based on Structure Analyses by Cryo-EM. CryoEM Symposium 2018.01.02 東京

  25. 藤吉好則. 構造生物学を激変させたクライオ電子顕微鏡法. CSJ化学フェスタ2017 2017.10.17 東京

  26. 藤吉好則. 構造生物学を激変させたCryoEM. 微細構造解析プラットフォームシンポジウム 2017.10.03 東京

  27. 藤吉好則. Structural Physiology of membrane proteins. International Cryo-EM symposium 2017.09.04 Netherland

  28. 藤吉好則. For effective structure analyses of membrane proteins. Symposium to celebrate Gunnar 2017.09.02 Sweden

  29. 藤吉好則. 創薬に役立つCryoEM. 第30回バイオメディカル分析化学シンポジウム 2017.08.29 東京

  30. 藤吉好則. Structural studies of membrane proteins by cyro-EM. 第17回日本蛋白質科学会年会 2017.06.20 仙台

  31. 藤吉好則. 膜タンパク質の構造生理学. 日本膜学会第39年会 2017.05.26 東京

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受賞学術賞 【 表示 / 非表示

  • 瑞宝重光章,2021年11月

  • 日本学士院会員(第2部第7分科(医学・薬学・歯学)),2019年12月

  • 藤原賞,2016年

  • Christian B. Anfinsen Award,2010年

  • 学士院賞,2008年

  • 紫綬褒章,2006年

  • 島津賞,2006年

  • 産学官連携功労者表彰 科学技術政策担当大臣賞,2005年

  • 山崎貞一賞,2005年

  • 慶應医学賞,2005年

  • クルージュナポカ医科薬科大学 名誉博士,2005年

  • 瀬藤賞,1988年

▼全件表示

 

社会貢献活動 【 表示 / 非表示

  • LF対談「クライオ電子顕微鏡を使う研究者は 世界でも日本でもとても増えています」,千里ライフサイエンス振興財団,Senri Life Science News No.92,2020年11月27日

  • 構造生理学について/クライオ電顕について,NHK,2019年06月25日