Takahashi J, Mizutani T, Sugihara HY, Nagata S, Kato S, Hiraguri Y, Takeoka S, Tsuchiya M, Kuno R, Kakinuma S, Watanabe M, Okamoto R. Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids. Cell Reports Methods. 2022.11; 2 (11): 100337. ( PubMed, DOI )

Rao C, Frodyma DE, Southekal S, Svoboda RA, Black AR, Guda C, Mizutani T, Clevers H, Johnson KR, Fisher KW, Lewis RE. KSR1- and ERK-dependent translational regulation of the epithelial-to-mesenchymal transition. eLife. 2021.05; 10 ( PubMed, DOI )

Reiko Kuno, Go Ito, Ami Kawamoto, Yui Hiraguri, Hady Yuki Sugihara, Sayaka Takeoka, Sayaka Nagata, Junichi Takahashi, Mao Tsuchiya, Sho Anzai, Tomohiro Mizutani, Hiromichi Shimizu, Shiro Yui, Shigeru Oshima, Kiichiro Tsuchiya, Mamoru Watanabe, Ryuichi Okamoto. Notch and TNF-α signaling promote cytoplasmic accumulation of OLFM4 in intestinal epithelium cells and exhibit a cell protective role in the inflamed mucosa of IBD patients. Biochem Biophys Rep. 2021.03; 25 100906. ( PubMed, DOI )

Tsuchiya Mao, Ito Go, Hama Minami, Nagata Sayaka, Kawamoto Ami, Suzuki Kohei, Shimizu Hiromichi, Anzai Sho, Takahashi Junichi, Kuno Reiko, Takeoka Sayaka, Hiraguri Yui, Sugihara Hady Yuki, Mizutani Tomohiro, Yui Shiro, Oshima Shigeru, Tsuchiya Kiichiro, Watanabe Mamoru, Okamoto Ryuichi. Functional analysis of isoflavones using patient-derived human colonic organoids BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2021.02; 542 40-47. ( PubMed, DOI )

Morikawa Ryo, Nemoto Yasuhiro, Yonemoto Yuki, Tanaka Shohei, Takei Yuria, Oshima Shigeru, Nagaishi Takashi, Tsuchiya Kiichiro, Nozaki Kengo, Mizutani Tomohiro, Nakamura Tetsuya, Watanabe Mamoru, Okamoto Ryuichi. Intraepithelial Lymphocytes Suppress Intestinal Tumor Growth by Cell-to-Cell Contact via CD103/E-Cadherin Signal CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY. 2021; 11 (5): 1483-1503. ( PubMed, DOI )

Yong-Hee Cho, Eun Ji Ro, Jeong-Su Yoon, Tomohiro Mizutani, Dong-Woo Kang, Jong-Chan Park, Tae Il Kim, Hans Clevers, Kang-Yell Choi. 5-FU promotes stemness of colorectal cancer via p53-mediated WNT/β-catenin pathway activation. Nat Commun. 2020.10; 11 5321.

Cho YH, Ro EJ, Yoon JS, Mizutani T, Kang DW, Park JC, Il Kim T, Clevers H, Choi KY. 5-FU promotes stemness of colorectal cancer via p53-mediated WNT/β-catenin pathway activation. Nature communications. 2020.10; 11 (1): 5321. ( PubMed, DOI )

Tanaka N, Kanatani S, Kaczynska D, Fukumoto K, Louhivuori L, Mizutani T, Kopper O, Kronqvist P, Robertson S, Lindh C, Kis L, Pronk R, Niwa N, Matsumoto K, Oya M, Miyakawa A, Falk A, Hartman J, Sahlgren C, Clevers H, Uhlén P. Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies. Nature biomedical engineering. 2020.09; 4 (9): 875-888. ( PubMed, DOI )

Nobuyuki Tanaka, Shigeaki Kanatani, Dagmara Kaczynska, Keishiro Fukumoto, Lauri Louhivuori, Tomohiro Mizutani, Oded Kopper, Pauliina Kronqvist, Stephanie Robertson, Claes Lindh, Lorand Kis, Robin Pronk, Naoya Niwa, Kazuhiro Matsumoto, Mototsugu Oya, Ayako Miyakawa, Anna Falk, Johan Hartman, Cecilia Sahlgren, Hans Clevers, Per Uhlén. Three-dimensional single-cell imaging for the analysis of RNA and protein expression in intact tumour biopsies. Nat Biomed Eng. 2020.06; 4 875-888.

Tomohiro Mizutani, Hans Clevers. Primary intestinal epithelial organoid culture. Methods Mol Biol. 2020.06; 2171 185-200.

Cayetano Pleguezuelos-Manzano, Jens Puschhof, Axel Rosendahl Huber, Arne van Hoeck, Henry M Wood, Jason Nomburg, Carino Gurjao, Freek Manders, Guillaume Dalmasso, Paul B Stege, Fernanda L Paganelli, Maarten H Geurts, Joep Beumer, Tomohiro Mizutani, Yi Miao, Reinier van der Linden, Stefan van der Elst, K Christopher Garcia, Janetta Top, Rob JL Willems, Marios Giannakis, Richard Bonnet, Phil Quirke, Matthew Meyerson, Edwin Cuppen, Ruben van Boxtel, Hans Clevers. Mutational signature in colorectal cancer caused by genotoxic pks+ E. coli. Nature. 2020.04; 580 269-273.

Pleguezuelos-Manzano C, Puschhof J, Rosendahl Huber A, van Hoeck A, Wood HM, Nomburg J, Gurjao C, Manders F, Dalmasso G, Stege PB, Paganelli FL, Geurts MH, Beumer J, Mizutani T, Miao Y, van der Linden R, van der Elst S, Genomics England Research Consortium., Garcia KC, Top J, Willems RJL, Giannakis M, Bonnet R, Quirke P, Meyerson M, Cuppen E, van Boxtel R, Clevers H. Mutational signature in colorectal cancer caused by genotoxic pks⁺ E. coli. Nature. 2020.04; 580 (7802): 269-273. ( PubMed, DOI )

Mizutani T, Clevers H. Primary Intestinal Epithelial Organoid Culture. Methods in molecular biology (Clifton, N.J.). 2020; 2171 185-200. ( PubMed, DOI )

Joosten SPJ, Mizutani T, Spaargaren M, Clevers H, Pals ST. MET Signaling Overcomes Epidermal Growth Factor Receptor Inhibition in Normal and Colorectal Cancer Stem Cells Causing Drug Resistance. Gastroenterology. 2019.10; 157 (4): 1153-1155.e1. ( PubMed, DOI )

Sander PJ Joosten, Tomohiro Mizutani, Marcel Spaargaren, Hans Clevers, Steven T Pals. MET signaling overcomes epidermal growth factor receptor inhibition in normal and colorectal cancer stem cells causing drug resistance. Gastroenterology. 2019.06; 157 1153-1155.

Fumiaki Ishibashi, Hiromichi Shimizu, Toru Nakata, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Sho Anzai, Reiko Kuno, Sayaka Nagata, Go Ito, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe, Ryuichi Okamoto. Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium. [Epub ahead of print] Stem Cell Reports. 2017.12; 10 (1): 27-42. ( PubMed, DOI )

Jarno Drost, Ruben van Boxtel, Francis Blokzijl, Tomohiro Mizutani, Nobuo Sasaki, Valentina Sasselli, Joep de Ligt, Sam Behjati, Judith E Grolleman, Tom van Wezel, Serena Nik-Zainal, Roland P Kuiper, Edwin Cuppen, Hans Clevers. Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer Science. 2017.09; 358 (6360): 234-238. ( PubMed, DOI )

Tomohiro Mizutani, Yoshiyuki Tsukamoto, Hans Clevers. Oncogene-inducible organoids as a miniature platform to assess cancer characteristics. J. Cell Biol. 2017.06; 216 (6): 1505-1507. ( PubMed, DOI )

Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, Ryuichi Okamoto. Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine. Data Brief. 2017.02; 10 551-556. ( PubMed, DOI )

Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, Ryuichi Okamoto. Indispensable role of Notch ligand-dependent signaling in the proliferation and stem cell niche maintenance of APC-deficient intestinal tumors. Biochem. Biophys. Res. Commun. 2017.01; 482 (4): 1296-1303. ( PubMed, DOI )

Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Toru Nakata, Tatsuro Murano, Go Ito, Hiromichi Shimizu, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Akihiro Araki, Kazuo Ohtsuka, Ryuichi Okamoto, Mamoru Watanabe. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells. Sci Rep. 2016.11; 6 36795. ( PubMed, DOI )

Taichi Matsumoto, Wakana Mochizuki, Yoichi Nibe, Shintaro Akiyama, Yuka Matsumoto, Kengo Nozaki, Masayoshi Fukuda, Ayumi Hayashi, Tomohiro Mizutani, Shigeru Oshima, Mamoru Watanabe, Tetsuya Nakamura. Retinol Promotes In Vitro Growth of Proximal Colon Organoids through a Retinoic Acid-Independent Mechanism. PLoS ONE. 2016.08; 11 (8): e0162049. ( PubMed, DOI )

Kengo Nozaki, Wakana Mochizuki, Yuka Matsumoto, Taichi Matsumoto, Masayoshi Fukuda, Tomohiro Mizutani, Mamoru Watanabe, Tetsuya Nakamura. Co-culture with intestinal epithelial organoids allows efficient expansion and motility analysis of intraepithelial lymphocytes. J. Gastroenterol. 2016.03; 51 (3): 206-213. ( PubMed, DOI )

Nobukatsu Horita, Kiichiro Tsuchiya, Ryohei Hayashi, Keita Fukushima, Shuji Hibiya, Masayoshi Fukuda, Yoshihito Kano, Tomohiro Mizutani, Yasuhiro Nemoto, Shiro Yui, Ryuichi Okamoto, Tetsuya Nakamura, Mamoru Watanabe. Fluorescent labelling of intestinal epithelial cells reveals independent long-lived intestinal stem cells in a crypt. Biochem Biophys Res Commun. 2014.10; 454 (4): 493-499. ( PubMed, DOI )

Masayoshi Fukuda, Tomohiro Mizutani, Wakana Mochizuki, Taichi Matsumoto, Kengo Nozaki, Yuriko Sakamaki, Shizuko Ichinose, Yukinori Okada, Toshihiro Tanaka, Mamoru Watanabe, Tetsuya Nakamura. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon. Genes Dev. 2014.08; 28 (16): 1752-1757. ( PubMed, DOI )

Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki, Tatsuro Murano, Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe. Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine. PeerJ. 2014.05; 2 (1): e370. ( PubMed, DOI )

Go Ito, Ryuichi Okamoto, Tatsuro Murano, Hiromichi Shimizu, Satoru Fujii, Toru Nakata, Tomohiro Mizutani, Shiro Yui, Junko Akiyama-Morio, Yasuhiro Nemoto, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe. Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells. PLoS ONE. 2013.11; 8 (11): e79693. ( PubMed, DOI )

Tomohiro Mizutani, Tetsuya Nakamura, Ryo Morikawa, Masayoshi Fukuda, Wakana Mochizuki, Yuhki Yamauchi, Kengo Nozaki, Shiro Yui, Yasuhiro Nemoto, Takashi Nagaishi, Ryuichi Okamoto, Kiichiro Tsuchiya, Mamoru Watanabe. Real-time analysis of P-glycoprotein-mediated drug transport across primary intestinal epithelium three-dimensionally cultured in vitro. Biochem. Biophys. Res. Commun.. 2012.03; 419 (2): 238-243. ( PubMed, DOI )

Shiro Yui, Tetsuya Nakamura, Toshiro Sato, Yasuhiro Nemoto, Tomohiro Mizutani, Xiu Zheng, Shizuko Ichinose, Takashi Nagaishi, Ryuichi Okamoto, Kiichiro Tsuchiya, Hans Clevers, Mamoru Watanabe. Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell. Nat. Med.. 2012.03; 18 (4): 618-623. ( PubMed, DOI )

Takahashi, Junichi, Mizutani, Tomohiro ,Okamoto, Ryuichi. A NOVEL SUSPENSION METHOD FOR EFFICIENT INDUCTION AND RAPID MATURATION OF HUMAN INTESTINAL ORGANOIDS USING A ROTATIONAL BIOREACTOR. DDW2022 2022.05

Ishibashi F, Shimizu H, Nakata T, Fujii S, Suzuki K, Kawamoto A, Anzai S, Kuno R, Nagata S, Ito G, Murano T, Mizutani T, Oshima S, Tsuchiya K, Nakamura T, Okamoto R, Watanabe M. Lineage tracing reveals contribution of atoh1+ cell-derived stem cells in the homeostasis, repair, and carcinogenesis of the intestinal epithelium. DDW2017 2017.05.09 Chicago(USA)

Minami Hama, Ayumi Hayashi, Tomohiro Mizutani, Satoru Fujii, Tatsuro Murano, Kiichiro Tsuchiya, Tetsuya Nakamura, Ken Watanabe, Norio Shimizu, Ryuichi Okamoto, Mamoru Watanabe. Prevalence of viral DNA in the mucosal tissue and cultured organoids of inflammatory bowel disease patients.. AOCC2016 2016.07.08 Kyoto International Conference Center, Kyoto（JAPAN）

Fujii, Satoru , Okamoto, Ryuichi , Nakata, Toru , Suzuki, Kohei , Ishibashi, Fumiaki, Kawamoto, Ami , Segawa, Sayaka Ohashi, Mizutani, Tomohiro , Tsuchiya, Kiichiro, Nakamura, Tetsuya , Watanabe, Mamoru . Establishment of a 3D cell culture-based screening platform to identify natural products that can regulate transepithelial water transport of the human gastrointestinal tract. Pacifichem2015 2015.12.17 Hawaii

Nobuyuki Horita, Kiichiro Tsuchiya, Ryohei Hayashi, Keita Fukushima, Shuji Hibiya, Masayoshi Fukuda, Tomohiro Mizutani, Ryuichi Okamoto, Testuya Nakamura and Mamoru Watanabe. A novel fluorescent labelling system into small intestinal organoid reveals independent long-lived intestinal stem cells in a crypt.. AOCC2015 2015.06.19 Beijing

Kohei Suzuki,Satoru　Fujii,Toru Nakata,Ami Kawamoto,Fumiaki Ishibashi,Sayaka Ohashi Segawa, Tomohiro Mizutani, Kiichiro Tsuchiya,Kazuo Otsuka,Tetsuya　Nakamura,　Ryuichi　Okamoto,　Mamoru Watanabe . Enrichment of Intestinal Stem Cells by the 3D-Culture of IBD Patient Derived Intestinal epithelium. AOCC2015 2015.06.19 Beijing

Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki, Tatsuro Murano, Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe. Distinct Expression Patterns of Notch Ligands, DLL1 and DLL4, in Normal and Inflamed Mice Intestine. The 2nd Annual Meeting of Asian Organization for Crohn's and Colitis 2014.06.21 Seoul

Mizutani T. Successful Engraftment of Cultured Small Intestinal Epithelial Stem Cells onto Damaged Colonic Mucosa by Heterotopic Transplantation. GI Research Academy 2014 2014.06.06 Tokyo

Mizutani T, Fukuda M, Mochizuki W, Matsumoto T, Nozaki K, Ichinose S, Watanabe M, Nakamura T. Successful Engraftment of Cultured Small Intestinal Epithelial Stem Cells onto Damaged Colonic Mucosa by Heterotopic Transplantation. Digestive Disease Week 2014 2014.05.03 Chicago

岡本隆一,村野竜朗,伊藤　剛,中田　徹,日比谷秀爾,藤井　悟,清水寛路,水谷知裕,土屋輝一郎,中村哲也,渡辺　守. 炎症性腸疾患におけるNotch-Hes1シグナルを介した粘膜再生機構の解析. 浜名湖シンポジウム 2013.12.21 浜松

Mizutani T, Nakamura T, Morikawa R, Fukuda M, Mochizuki W, Yamauchi Y, Nozaki K, Yui S, Okamoto R, Tsuchiya K, Watanabe M. Real-time analysis of p-glycoprotein-mediated drug transport across primary intestinal epitherial cells three-dimensionally cultured in vitro. DDW 2012 2012.05.19 San Diego