Personnel Information

写真a

SAITO Kenta


Job title

Junior Associate Professor

Campus Career 【 display / non-display

  • 2012.07
    -
    2018.03
    Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Medical and Dental Sciences, Cognitive and Behavioral Medicine, Neuroanatomy and Cellular Neurobiology, Assistant Professor
  • 2018.04
    -
    2021.03
    Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Medical and Dental Sciences, Cognitive and Behavioral Medicine, Neuroanatomy and Cellular Neurobiology, Assistant Professor
  • 2021.04
    -
    Now
    Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Medical and Dental Sciences, Cognitive and Behavioral Medicine, Neuroanatomy and Cellular Neurobiology, Junior Associate Professor
 

Published Papers & Misc 【 display / non-display

  1. Ayana Sugizaki, Keisuke Sato, Kazuyoshi Chiba, Kenta Saito, Masahiko Kawagishi, Yuri Tomabechi, Shalin B Mehta, Hirokazu Ishii, Naoki Sakai, Mikako Shirouzu, Tomomi Tani, Sumio Terada. POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos Proceedings of the National Academy of Sciences of the United States of America. 2021.03; 118 (11): ( DOI )

  2. Nori Nakai, Keisuke Sato, Tomomi Tani, Kenta Saito, Fumiya Sato, and Sumio Terada. Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy Microscopy. 2019; ( DOI )

  3. Kenta Saito, Masafumi Nakano,Takeharu Nagai. Luminescence Imaging: (a) Multicolor Visualization of Ca2+ Dynamics in Different Cellular Compartments and (b) Video-Rate Tumor Detection in a Freely Moving Mouse. Meth. Mol. Biol.. 2016.01; 1461 289-297. ( DOI )

  4. Masahito Yamanaka, Kenta Saito, Nicholas I Smith, Yoshiyuki Arai, Kumiko Uegaki, Yasuo Yonemaru, Kentaro Mochizuki, Satoshi Kawata, Takeharu Nagai, Katsumasa Fujita. Visible-wavelength two-photon excitation microscopy for fluorescent protein imaging. J Biomed Opt. 2015.10; 20 (10): 101202. ( PubMed, DOI )

  5. Kenta Saito, Takeharu Nagai. Recent progress in luminescent proteins development. Curr Opin Chem Biol. 2015.08; 27 46-51. ( PubMed, DOI )

  6. Akira Takai, Masahiro Nakano, Kenta Saito, Remi Haruno, Tomonobu M Watanabe, Tatsuya Ohyanagi, Takashi Jin, Yasushi Okada, Takeharu Nagai. Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A.. 2015.04; 112 (14): 4352-4356. ( PubMed, DOI )

  7. Takeharu Nagai, Kazuki Horikawa, Kenta Saito, Tomoki Matsuda. Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics. Front Mol Neurosci. 2014.11; 7 90. ( PubMed, DOI )

  8. Masahito Yamanaka, Kenta Saito, Nicholas I Smith, Satoshi Kawata, Takeharu Nagai, Katsumasa Fujita. Saturated excitation of fluorescent proteins for subdiffraction-limited imaging of living cells in three dimensions Interface focus: a theme supplement of Journal of the Royal Society interface. 2013.10; 3 (5): 20130007.

  9. Tomoki Matsuda, Kazuki Horikawa, Kenta Saito, Takeharu Nagai. Highlighted Ca2+ imaging with a genetically encoded 'caged' indicator Scientific Reports. 2013.03; 3 1398.

  10. 永井健治、齊藤健太、初谷紀幸. 化学発光タンパク質の高輝度化とバイオイメージングへの展開 顕微鏡. 2013; 48 1-3.

  11. K. Saito, Y. Higuchi, Y. Arai, T. Nagai. Video-rate imaging of luminescent tumour cells in freely moving unshaved mice. Protoc. Exch.. 2013; ( DOI )

  12. Kenta Saito, Y-F Chang, Kazuki Horikawa, Noriyuki Hatsugai, Yuriko Higuchi, Mitsuru Hashida, Yu Yoshida, Tomoki Matsuda, Yoshiyuki Arai, Takeharu Nagai. Luminescent proteins for high-speed single-cell and whole-body imaging. Nature communications. 2012.12; 3 1262. ( PubMed, DOI )

  13. Saito K, Arai Y, Zhang J, Kobayashi K, Tani T, Nagai T. Conjugation of both on-axis and off-axis light in Nipkow disk confocal microscope to increase availability of incoherent light source. Cell structure and function. 2011; 36 237-246. ( PubMed )

  14. Sadamoto H, Saito K, Muto H, Kinjo M, Ito E. Direct observation of dimerization between different CREB1 isoforms in a living cell. PloS one. 2011; 6 e20285. ( PubMed, DOI )

  15. Saito K, Hatsugai N, Horikawa K, Kobayashi K, Matsu-Ura T, Mikoshiba K, Nagai T. Auto-luminescent genetically-encoded ratiometric indicator for real-time Ca2+ imaging at the single cell level. PloS one. 2010.04; 5 e9935. ( PubMed, DOI )

  16. Tomosugi W, Matsuda T, Tani T, Nemoto T, Kotera I, Saito K, Horikawa K, Nagai T. An ultramarine fluorescent protein with increased photostability and pH insensitivity. Nature methods. 2009.05; 6 351-353. ( PubMed, DOI )

  17. Yamamoto T, Kumagai A, Saito K, Nagai T. Two-photon excitation behavior of thiophene-based oligomers and a polymer. Journal of nanoscience and nanotechnology. 2009.01; 9 670-672. ( PubMed )

  18. Imamura, H. and Nhat, K. P. H. and Togawa, H. and Saito, K. and Iino, R. and Kato-Yamada, Y. and Nagai, T. and Noji, H.. Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators Proc. Natl. Acad. Sci. U.S.A.. 2009; 106 (37): 15651-15656.

  19. Saito K, Kobayashi K, Tani T, Nagai T. A mercury arc lamp-based multi-color confocal real time imaging system for cellular structure and function. Cell structure and function. 2008; 33 133-141. ( PubMed )

  20. Oguma, T. and Hosaka, S. and Katayama, T. and Matsuda, T. and Saito, K. and Nagai, T. and Minami, M.. Effects of astrocyte morphological changes on brain cell function Yakugaku Zasshi-Journal of the Pharmaceutical Society of Japan. 2008; 128 192-195.

  21. 福岡 創, 齋藤 健太, 永井 健治, 本間 道夫, 石島 秋彦. 3P179 イオン駆動回転モーター構成素子のGFPによる一分子標識(分子モーター,ポスター発表,第45回日本生物物理学会年会) 生物物理. 2007.11; 47 (1):

  22. 今村 博臣, Kim Huynh Nhat Phuong, 齊藤 健太, 飯野 亮太, 山田 康之, 永井 健治, 野地 博行. 3P326 細胞内ATPを蛍光で可視化する(バイオイメージング,ポスター発表,第45回日本生物物理学会年会) 生物物理. 2007.11; 47 (1):

  23. 谷 知己, 齋藤 健太, 永井 健治. 3P323 リガンド結合にともなう神経成長因子受容体TrkAの構造ダイナミクスを可視化する試み(バイオイメージング,ポスター発表,第45回日本生物物理学会年会) 生物物理. 2007.11; 47 (1):

  24. 友杉 亘, 松田 知己, 小寺 一平, 齋藤 健太, 永井 健治. 3P305 群青色蛍光蛋白質の開発(バイオイメージング,ポスター発表,第45回日本生物物理学会年会) 生物物理. 2007.11; 47 (1):

  25. Ohsugi Y, Saito K, Tamura M, Kinjo M. Lateral mobility of membrane-binding proteins in living cells measured by total internal reflection fluorescence correlation spectroscopy. Biophysical journal. 2006.11; 91 3456-3464. ( PubMed, DOI )

  26. Pack C, Saito K, Tamura M, Kinjo M. Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs. Biophysical journal. 2006.11; 91 3921-3936. ( PubMed, DOI )

  27. Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A. A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nature biotechnology. 2006.05; 24 577-581. ( PubMed, DOI )

  28. Lakowicz, Joseph R.. Chapter 13 Energy Transfer. In: PRINCIPLES OF FLUORESCENCE SPECTROSCOPY 2006;

  29. Ohsugi, Y. and Saito, K. and Tamura, M. and Kinjo, M.. Development of a novel monitoring system for cell activity based on visualization of chromosome dynamics Cell Structure and Function. 2005; 30 117.

  30. Watanabe K, Saito K, Kinjo M, Matsuda T, Tamura M, Kon S, Miyazaki T, Uede T. Molecular dynamics of STAT3 on IL-6 signaling pathway in living cells. Biochemical and biophysical research communications. 2004.11; 324 1264-1273. ( PubMed, DOI )

  31. Saito K, Wada I, Tamura M, Kinjo M. Direct detection of caspase-3 activation in single live cells by cross-correlation analysis. Biochemical and biophysical research communications. 2004.11; 324 849-854. ( PubMed, DOI )

  32. Földes-Papp Z, Costa JM, Demel U, Tilz GP, Kinjo M, Saito K, Kii H, Takagi T, Tamura M, Thyberg P, Birch-Hirschfeld E. Specifically associated PCR products probed by coincident detection of two-color cross-correlated fluorescence intensities in human gene polymorphisms of methylene tetrahydrofolate reductase at site C677T: a novel measurement approach without follow-up ma Experimental and molecular pathology. 2004.06; 76 212-218. ( PubMed, DOI )

  33. Sadamoto, H. and Wagatsuma, A. and Saito, K. and Kinjo, M. and Ito, E.. CREB isoforms in central nervous system of pond snail Lymnaea stagnalis Zoological Science. 2004; 21 (12): 1317.

  34. Saito K, Ito E, Takakuwa Y, Tamura M, Kinjo M. In situ observation of mobility and anchoring of PKCbetaI in plasma membrane. FEBS letters. 2003.04; 541 126-131. ( PubMed )

  35. Földes-Papp Z, Kinjo M, Saito K, Kii H, Takagi T, Tamura M, Costa JM, Birch-Hirschfeld E, Demel U, Thyberg P, Tilz GP. C677T single nucleotide polymorphisms of the human methylene tetrahydrofolate reductase and specific identification : a novel strategy using two-color cross-correlation fluorescence spectroscopy. Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology. 2003; 7 99-111. ( PubMed )

  36. Muto, T. and Saito, K. and Tamura, M. and Kinjo, M.. Microenvironment analysis in squid axons using fluorescence correlation spectroscopy and laser scanning microscopy Acta Histochemica Et Cytochemica. 2002; 35 (2): 87-91.

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Conference Activities & Talks 【 display / non-display

  1. Keisuke Sato, Nori Nakai-Kadowaki, Ayana Sugizaki, Kazuyoshi Chiba, Kenta Saito, Masahiko Kawagishi, Yuri Tomabechi, Shalin B. Mehta, Hirokazu Ishii, Naoki Sakai, Hiromasa Ka, Mikako Shirouzu, Tomomi Tani, Sumio Terada. Development of POLArIS, a versatile probe for multi-color/multi-target orientation imaging in living cells. Cell Bio 2022 2022.12.04 Washington, DC

  2. Nori Nakai, Keisuke Sato, Tomomi Tani, Masahiko Kawagishi, Hiromasa Ka, Kenta Saito, Sumio Terada. EXPANDED REPERTOIRE OF POLARIS, A VERSATILE FLUORESCENT PROBE FOR MOLECULAR ORIENTATION. Biophysical Society 66th Annual Meeting 2022.02.23 USA

  3. 佐藤啓介, 杉﨑 綾奈, 齊藤 健太, Shalin B. Mehta, 白水 美香子, 谷 知己, 寺田 純雄. F-actin特異的分子配向プローブPOLArISactの開発と評価. 第126回日本解剖学会総会・全国学術集会 2021.03.29 オンライン開催