Tomohiko Murakami, Yoshifumi Takahata, Kenji Hata, Kosuke Ebina, Katsutoshi Hirose, Lerdluck Ruengsinpinya, Yuri Nakaminami, Yuki Etani, Sachi Kobayashi, Takashi Maruyama, Hiroyasu Nakano, Takehito Kaneko, Satoru Toyosawa, Hiroshi Asahara, Riko Nishimura. Semaphorin 4D induces articular cartilage destruction and inflammation in joints by transcriptionally reprogramming chondrocytes. Sci Signal. 2022.11; 15 (758): eabl5304. ( PubMed, DOI )

Takayoshi Sasako, Toshihiro Umehara, Kotaro Soeda, Kazuma Kaneko, Miho Suzuki, Naoki Kobayashi, Yukiko Okazaki, Miwa Tamura-Nakano, Tomoki Chiba, Domenico Accili, C Ronald Kahn, Tetsuo Noda, Hiroshi Asahara, Toshimasa Yamauchi, Takashi Kadowaki, Kohjiro Ueki. Deletion of skeletal muscle Akt1/2 causes osteosarcopenia and reduces lifespan in mice. Nat Commun. 2022.10; 13 (1): 5655. ( PubMed, DOI )

Ryo Nakamichi, Shang Ma, Takayuki Nonoyama, Tomoki Chiba, Ryota Kurimoto, Hiroki Ohzono, Merissa Olmer, Chisa Shukunami, Noriyuki Fuku, Guan Wang, Errol Morrison, Yannis P Pitsiladis, Toshifumi Ozaki, Darryl D'Lima, Martin Lotz, Ardem Patapoutian, Hiroshi Asahara. The mechanosensitive ion channel PIEZO1 is expressed in tendons and regulates physical performance. Sci Transl Med. 2022.06; 14 (647): eabj5557. ( PubMed, DOI )

Yuta Fujii, Lin Liu, Lisa Yagasaki, Maiko Inotsume, Tomoki Chiba, Hiroshi Asahara. Cartilage Homeostasis and Osteoarthritis. Int J Mol Sci. 2022.06; 23 (11): ( PubMed, DOI )

Manabu Kawata, Takeshi Teramura, Philip Ordoukhanian, Steven R Head, Padmaja Natarajan, Aishwarya Sundaresan, Merissa Olmer, Hiroshi Asahara, Martin K Lotz. Krüppel-like factor-4 and Krüppel-like factor-2 are important regulators of joint tissue cells and protect against tissue destruction and inflammation in osteoarthritis. Ann Rheum Dis. 2022.05; ( PubMed, DOI )

Kaho Takada, Tomoki Chiba, Takayuki Miyazaki, Lisa Yagasaki, Ryo Nakamichi, Takanori Iwata, Keiji Moriyama, Hiroyuki Harada, Hiroshi Asahara. Single Cell RNA Sequencing Reveals Critical Functions of Mkx in Periodontal Ligament Homeostasis Front Cell Dev Biol. 2022.02; 10 795441. ( PubMed, DOI )

Hiroki Tsutsumi, Ryota Kurimoto, Ryo Nakamichi, Tomoki Chiba, Takahide Matsushima, Yuta Fujii, Risa Sanada, Tomomi Kato, Kana Shishido, Yuriko Sakamaki, Tsuyoshi Kimura, Akio Kishida, Hiroshi Asahara. Generation of a tendon-like tissue from human iPS cells. J Tissue Eng. 2022.01; 13 20417314221074018. ( PubMed, DOI )

Yoshiaki Ito, Tokio Matsuzaki, Fumiaki Ayabe, Sho Mokuda, Ryota Kurimoto, Takahide Matsushima, Yusuke Tabata, Maiko Inotsume, Hiroki Tsutsumi, Lin Liu, Masahiro Shinohara, Yoko Tanaka, Ryo Nakamichi, Keiichiro Nishida, Martin K Lotz, Hiroshi Asahara. Both microRNA-455-5p and -3p repress hypoxia-inducible factor-2α expression and coordinately regulate cartilage homeostasis. Nat Commun. 2021.07; 12 (1): 4148. ( PubMed, DOI )

Takayuki Miyazaki, Ryota Kurimoto, Tomoki Chiba, Takahide Matsushima, Ryo Nakamichi, Hiroki Tsutsumi, Kaho Takada, Lisa Yagasaki, Tomomi Kato, Kana Shishido, Yukiho Kobayashi, Tsutomu Matsumoto, Keiji Moriyama, Hiroshi Asahara. Mkx regulates the orthodontic tooth movement via osteoclast induction. J Bone Miner Metab. 2021.05; 39 (5): 780-786. ( PubMed, DOI )

Yutaro Uchida, Takahide Matsushima, Ryota Kurimoto, Tomoki Chiba, Yuki Inutani, Hiroshi Asahara. Identification of chemical compounds regulating PD-L1 by introducing HiBiT-tagged cells. FEBS Lett. 2021.03; 595 (5): 563-576. ( PubMed, DOI )

Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Yoshiaki Ito, Ryo Nakamichi, Martin Lotz, Hiroshi Asahara. MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes. Methods Mol Biol. 2021; 2245 151-166. ( PubMed, DOI )

Keiko Itano,KojiOchiai, Takahide Matsushima,Hiroshi Asahara and Koichi Takahashi. Automation of Foci-Cell-State Judgement with Regression Models Transactions of the Institute of Systems, Control and Information Engineers. 2021; 34 (3): 69-80. ( DOI )

Kwang Il Lee, Ramya Gamini, Merissa Olmer, Yasunari Ikuta, Joe Hasei, Jihye Baek, Oscar Alvarez-Garcia, Shawn P Grogan, Darryl D D'Lima, Hiroshi Asahara, Andrew I Su, Martin K Lotz. Mohawk is a transcription factor that promotes meniscus cell phenotype and tissue repair and reduces osteoarthritis severity. Sci Transl Med. 2020.10; 12 (567): 7967. ( PubMed, DOI )

Ryota Kurimoto, Tomoki Chiba, Yoshiaki Ito, Takahide Matsushima, Yuki Yano, Kohei Miyata, Yuka Yashiro, Tsutomu Suzuki, Kozo Tomita, Hiroshi Asahara. The tRNA pseudouridine synthase TruB1 regulates the maturation of let-7 miRNA. EMBO J. 2020.10; 39 (20): e104708. ( PubMed, DOI )

Ryo Nakamichi, Ryota Kurimoto, Yusuke Tabata, Hirosi Asahara. Transcriptional, epigenetic and microRNA regulation of growth plate. Bone. 2020.08; 137 115434. ( PubMed, DOI )

Ryo Nakamichi, Hiroshi Asahara. Regulation of tendon and ligament differentiation. Bone. 2020.08; 115609. ( PubMed, DOI )

Mokuda S, Nakamichi R, Ito Y, Asahara H. Genetic, Epigenetic, and MicroRNA Regulation of Osteoarthritis Encyclopedia of Bone Biology, vol.[1]. 2020.06; 1 641-651.

Tempei Sato, Kensuke Kataoka, Yoshiaki Ito, Shigetoshi Yokoyama, Masafumi Inui, Masaki Mori, Satoru Takahashi, Keiichi Akita, Shuji Takada, Hiroe Ueno-Kudoh, Hiroshi Asahara. Lin28a/let-7 pathway modulates the Hox code via Polycomb regulation during axial patterning in vertebrates Elife. 2020.05; 9 ( PubMed, DOI )

Ryo Nakamichi, Hiroshi Asahara. The transcription factors regulating intervertebral disc development. JOR Spine. 2020.03; 3 (1): e1081. ( PubMed, DOI )

Eric Gracey, Arne Burssens, Isabelle Cambré, Georg Schett, Rik Lories, Iain B McInnes, Hiroshi Asahara, Dirk Elewaut. Tendon and ligament mechanical loading in the pathogenesis of inflammatory arthritis. Nat Rev Rheumatol. 2020.02; 16 (4): 193-207. ( PubMed, DOI )

Yuki Yano, Tomoki Chiba, Hiroshi Asahara. Analysis of the Mouse Y Chromosome by Single-Molecule Sequencing With Y Chromosome Enrichment. Front Genet. 2020; 11 406. ( PubMed, DOI )

Kensuke Kataoka, Ryota Kurimoto, Hiroki Tsutsumi, Tomoki Chiba, Tomomi Kato, Kana Shishido, Mariko Kato, Yoshiaki Ito, Yuichiro Cho, Osamu Hoshi, Ayako Mimata, Yuriko Sakamaki, Ryo Nakamichi, Martin K Lotz, Keiji Naruse, Hiroshi Asahara. In vitro Neo-Genesis of Tendon/Ligament-Like Tissue by Combination of Mohawk and a Three-Dimensional Cyclic Mechanical Stretch Culture System Front Cell Dev Biol. 2020; 8 307. ( PubMed, DOI )

Hiroto Yamamoto, Yutaro Uchida, Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima, Maiko Inotsume, Chihiro Ishikawa, Haiyan Li, Takashi Shiga, Masafumi Muratani, Tokujiro Uchida, Hiroshi Asahara. Transcriptome analysis of sevoflurane exposure effects at the different brain regions. PLoS One. 2020; 15 (12): e0236771. ( PubMed, DOI )

Carmen Adriaens, Florian Rambow, Greet Bervoets, Toomas Silla, Mari Mito, Tomoki Chiba, Hiroshi Asahara, Tetsuro Hirose, Shinichi Nakagawa, Torben Heick Jensen, Jean-Christophe Marine. The long noncoding RNA NEAT1_1 is seemingly dispensable for normal tissue homeostasis and cancer cell growth. RNA. 2019.12; 25 (12): 1681-1695. ( PubMed, DOI )

Momo Isobe, Hikaru Toya, Mari Mito, Tomoki Chiba, Hiroshi Asahara, Tetsuro Hirose, Shinichi Nakagawa. Forced isoform switching of Neat1_1 to Neat1_2 leads to the loss of Neat1_1 and the hyperformation of paraspeckles but does not affect the development and growth of mice. RNA. 2019.12; ( PubMed, DOI )

Yutaro Uchida, Tomoki Chiba, Ryota Kurimoto, Hiroshi Asahara. Post-transcriptional regulation of inflammation by RNA-binding proteins via cis-elements of mRNAs. J. Biochem.. 2019.11; 166 (5): 375-382. ( PubMed, DOI )

Satoshi Yamashita, Kensuke Kataoka, Hiroto Yamamoto, Tomoko Kato, Satoshi Hara, Katsushi Yamaguchi, Claire Renard-Guillet, Yuki Katou, Katsuhiko Shirahige, Haruki Ochi, Hajime Ogino, Tokujiro Uchida, Masafumi Inui, Shuji Takada, Shuji Shigenobu, Hiroshi Asahara. Comparative analysis demonstrates cell type-specific conservation of SOX9 targets between mouse and chicken. Sci Rep. 2019.08; 9 (1): 12560. ( PubMed, DOI )

Kita M, Nakae J, Kawano Y, Asahara H, Takemori H, Okado H, Itoh H. Zfp238 Regulates the Thermogenic Program in Cooperation with Foxo1. iScience. 2019.02; 12 87-101. ( PubMed, DOI )

Takayoshi Sasako, Mitsuru Ohsugi, Naoto Kubota, Shinsuke Itoh, Yukiko Okazaki, Ai Terai, Tetsuya Kubota, Satoshi Yamashita, Kunio Nakatsukasa, Takumi Kamura, Kaito Iwayama, Kumpei Tokuyama, Hiroshi Kiyonari, Yasuhide Furuta, Junji Shibahara, Masashi Fukayama, Kenichiro Enooku, Kazuya Okushin, Takeya Tsutsumi, Ryosuke Tateishi, Kazuyuki Tobe, Hiroshi Asahara, Kazuhiko Koike, Takashi Kadowaki, Kohjiro Ueki. Hepatic Sdf2l1 controls feeding-induced ER stress and regulates metabolism. Nat Commun. 2019.02; 10 (1): 947. ( PubMed, DOI )

Mitsumura T, Ito Y, Chiba T, Matsushima T, Kurimoto R, Tanaka Y, Kato T, Uchida K, Ito T, Yamamoto K, Eishi Y, Kitagawa M, Miyazaki Y, Inase N, Asahara H. Ablation of miR-146b in mice causes hematopoietic malignancy Blood Advances. 2018.11; 2 (23): 3483-3491. ( PubMed, DOI )

Takeshi Saito, Satoshi Hara, Tomoko Kato, Moe Tamano, Akari Muramatsu, Hiroshi Asahara, Shuji Takada. A tandem repeat array in IG-DMR is essential for imprinting of paternal allele at the Dlk1-Dio3 domain during embryonic development. Hum. Mol. Genet.. 2018.09; 27 (18): 3283-3292. ( PubMed, DOI )

Yusuke Mochizuki, Tomoki Chiba, Kensuke Kataoka, Satoshi Yamashita, Tempei Sato, Tomomi Kato, Kenji Takahashi, Takeshi Miyamoto, Masashi Kitazawa, Tomohisa Hatta, Tohru Natsume, Shinro Takai, Hiroshi Asahara. Combinatorial CRISPR/Cas9 Approach to Elucidate a Far-Upstream Enhancer Complex for Tissue-Specific Sox9 Expression. Dev. Cell. 2018.08; 46 (6): 794-806.e6. ( PubMed, DOI )

Alvarez-Garcia O, Matsuzaki T, Olmer M, Miyata K, Mokuda S, Sakai D, Masuda K, Asahara H, Lotz MK. FOXO are required for intervertebral disk homeostasis during aging and their deficiency promotes disk degeneration. Aging Cell. 2018.07; e12800. ( PubMed, DOI )

Nakamichi R, Kataoka K, Asahara H. Essential role of Mohawk for tenogenic tissue homeostasis including spinal disc and periodontal ligament. Modern rheumatology. 2018.05; 1-8. ( PubMed, DOI )

Masafumi Inui, Sho Mokuda, Tempei Sato, Moe Tamano, Shuji Takada, Hiroshi Asahara. Dissecting the roles of miR-140 and its host gene. Nat. Cell Biol.. 2018.05; 20 (5): 516-518. ( PubMed, DOI )

Tokio Matsuzaki, Oscar Alvarez-Garcia, Sho Mokuda, Keita Nagira, Merissa Olmer, Ramya Gamini, Kohei Miyata, Yukio Akasaki, Andrew I Su, Hiroshi Asahara, Martin K Lotz. FoxO transcription factors modulate autophagy and proteoglycan 4 in cartilage homeostasis and osteoarthritis. Sci Transl Med. 2018.02; 10 (428): ( PubMed, DOI )

Kataoka K, Matsushima T, Ito Y, Sato T, Yokoyama S, Asahara H. Bhlha9 regulates apical ectodermal ridge formation during limb development. JBMM. 2018.01; 36 (1): 64-72. ( PubMed, DOI )

kuda K, Kobayashi S, Fukaya M, Watanabe A, Murakami T, Hagiwara M, Sato T, Ueno H, Ogonuki N, Komano-Inoue S, Manabe H, Yamaguchi M, Ogura A, Asahara H, Sakagami H, Mizuguchi M, Manabe T, Tanaka T. CDKL5 controls postsynaptic localization of GluN2B-containing NMDA receptors in the hippocampus and regulates seizure susceptibility. Neurobiol Dis. 2017.10; 106 158-170. ( PubMed, DOI )

Shiba D, Mizuno H, Yumoto A, Shimomura M, Kobayashi H, Morita H, Shimbo M, Hamada M, Kudo T, Shinohara M, Asahara H, Shirakawa M, Takahashi S.. Development of new experimental platform 'MARS'-Multiple Artificial-gravity Research System-to elucidate the impacts of micro/partial gravity on mice. Sci Rep. 2017.09; 7 (1): 10837. ( PubMed, DOI )

Asahara H*, Inui M*, Lotz M. Tendons and Ligaments: Connecting Developmental Biology to Musculoskeletal Disease Pathogenesis. JBMR. 2017.09; 32 (9): 1773-1782. ( PubMed )

Miyata K, Naito M, Miyata T, Mokuda S, Asahara H*. Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells. Methods Mol Biol. 2017.08; (1668): 3-13. ( PubMed )

Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M. Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin. J Neurosci. 2017.05; 37 (12): 3181-3191. ( PubMed, DOI )

Yokoyama S, Furukawa S, Kitada S, Mori M, Saito T, Kawakami K, Izpisua Belmonte JC, Kawakami Y, Ito Y, Sato T, AsaharaH. Analysis of transcription factors expressed at the anterior mouse limb bud. PLoS One. 2017.05; 12 (5): e0175673. ( PubMed, DOI )

Oba A, Shimada S, Akiyama Y, Nishikawaji T, Mogushi K, Ito H, Matsumura S, Aihara A, Mitsunori Y, Ban D, Ochiai T, Kudo A, Asahara H, Kaida A, Miura M, Tanabe M, Tanaka S. ARID2 modulates DNA damage response in human hepatocellular carcinoma cells. J Hepato. 2017.05; 66 (5): 942-951. ( PubMed, DOI )

Ito Y, Inoue A, Seers T, Hato Y, Igarashi A, Toyama T, Taganov K, Boldin M, AsaharaH. Identification of targets of tumor suppressor microRNA-34a using a reporter library system. Proc Natl Acad Sci U S A. 2017.04; 114 (15): 3927-3932. ( PubMed, DOI )

Nozomu Yachie, Robotic Biology Consortium, Tohru Natsume. Robotic crowd biology with Maholo LabDroids. Nat. Biotechnol.. 2017.04; 35 (4): 310-312. ( PubMed, DOI )

Matsui-Hasumi A, Sato Y, Uto-Konomi A, Yamashita S, Uehori J, Yoshimura A, Yamashita M, Asahara H, Suzuki S, Kubo M. E3 ubiquitin ligases SIAH1/2 regulate hypoxia-inducible factor-1 (HIF-1)-mediated Th17 cell differentiation. International immunology. 2017.03; 29 (3): 133-143. ( PubMed, DOI )

Saito T, Hara S, Tamano M, Asahara H, Takada S. Deletion of conserved sequences in IG-DMR at Dlk1-Gtl2 locus suggests their involvement in expression of paternally expressed genes in mice. J Reprod Dev. 2017.02; 63 (1): 101-109. ( PubMed, DOI )

Hasei J, Teramura T, Takehara T, Onodera Y, Horii T, Olmer M, Hatada I, Fukuda K, Ozaki T, Lotz M, Asahara H. TWIST1 induces MMP3 expression through up-regulating DNA hydroxymethylation and promotes catabolic responses in human chondrocytes. Sci Rep. 2017.02; 7 42990. ( PubMed, DOI )

Nakasuji T, Ogonuki N, Chiba T, Kato T, Shiozawa K, Yamatoya K, Tanaka H, Kondo T, Miyado K, Miyasaka N, Kubota T, Ogura A, Asahara H. . Complementary critical functions of Zfy1 and Zfy2 in mouse spermatogenesis and reproductiontran PLoS Genetics. 2017.01; ( PubMed, DOI )

Koda N, Sato T, Shinohara M, Ichinose S, Ito Y, Nakamichi R, Kayama T, Kataoka K, Suzuki H, Moriyama K, Asahara H. The transcription factor mohawk homeobox regulates homeostasis of the periodontal ligament. Development (Cambridge, England). 2017.01; 144 (2): 313-320. ( PubMed, DOI )

Otero M, Peng H, El Hachem K, Culley KL, Wondimu EB, Quinn J, Asahara H, Tsuchimochi K, Hashimoto K, Goldring MB. ELF3 modulates type II collagen gene (COL2A1) transcription in chondrocytes by Inhibiting SOX9-CBP/p300-driven histone acetyltransferase activity. Connect Tissue Res. 2017.01; 58 (1): 15-26. ( PubMed, DOI )

Asahara H*.. Current Status and Strategy of microRNA Research for Cartilage Development and Osteoarthritis Pathogenesis. J Bone Metab. . 2016.08; 23 (3): 121-127. ( PubMed, DOI )

Ryo Nakamichi, Yoshiaki Ito, Masafumi Inui, Naoko Onizuka, Tomohiro Kayama, Kensuke Kataoka, Hidetsugu Suzuki, Masaki Mori, Masayo Inagawa, Shizuko Ichinose, Martin K Lotz, Daisuke Sakai, Koichi Masuda, Toshifumi Ozaki, Hiroshi Asahara. Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs. Nat Commun. 2016.08; 7 12503. ( PubMed, DOI )

Naito M, Mori M, Inagawa M, Miyata K, Hashimoto N, Tanaka S, Asahara H.. Dnmt3a Regulates Proliferation of Muscle Satellite Cells via p57Kip2. PLoS Genetics. . 2016.07; 12 (7): e1006167. ( PubMed, DOI )

Shimbo M, Kudo T, Hamada M, Jeon H, Imamura Y, Asano K, Okada R, Tsunakawa Y, Mizuno S, Yagami KI, Ishikawa C, Li H, Shiga T, Ishida J, Hamada J, Murata K, Ishimaru T, Hashimoto M, Fukamizu A, Yamane M, Ikawa M, Morita H, Shinohara M, Asahara H, Akiyama T, Akiyama N, Sasanuma H, Yoshida N, Zhou R, Wang YY, Ito T, Kokubu Y, Noguchi TK, Ishimine H, Kurisaki A, Shiba D, Mizuno H, Shirakawa M, Ito N, Takeda S, Takahashi S.. Ground-based assessment of JAXA mouse habitat cage unit by mouse phenotypic studies. Experimental Animals. 2016.05; ( PubMed, DOI )

Kayama T, Mori M, Ito Y, Matsushima T, Nakamichi R, Suzuki H, Ichinose S, Saito M, Marumo K, Asahara H.. Gtf2ird1-dependent Mohawk (Mkx) expression regulates mechanosensing properties of tendon. Mol Cell Biol. 2016.02; ( PubMed, DOI )

Doiguchi M, Nakagawa T, Imamura Y, Yoneda M, Higashi M, Kubota K, Yamashita S, Asahara H, Iida M, Fujii S, Ikura T, Liu Z, Nandu T, Kraus WL, Ueda H, Ito T. SMARCAD1 is an ATP-dependent stimulator of nucleosomal H2A acetylation via CBP, resulting in transcriptional regulation Sci Rep. 2016.02; ( PubMed, DOI )

Hidetsugu Suzuki, Yoshiaki Ito, Masahiro Shinohara, Satoshi Yamashita, Shizuko Ichinose, Akio Kishida, Takuya Oyaizu, Tomohiro Kayama, Ryo Nakamichi, Naoki Koda, Kazuyoshi Yagishita, Martin K Lotz, Atsushi Okawa, Hiroshi Asahara. Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossification of Achilles tendon via failed tenogenesis. Proc. Natl. Acad. Sci. U.S.A.. 2016.01; 113 (28): 7840-7845. ( PubMed, DOI )

T. Chiba, Y. Ito, H. Asahara.. Post-Transcriptional Gene Regulation by MicroRNA and RNA-Binding Protein Biomedical Sciences. 2016;

Ito Y, Mokuda S, Miyata K, Matsushima T, Asahara H.. MicroRNAs in Chronic Inflammation Diseases. Chronic Inflammation (Chapter 6) 2016 Springer Japan. Review. Book.. 2016; ( DOI )

Ogura Y, Miyake N, Kou I, Iida A, Nakajima M, Takeda K, Fujibayashi S, Shiina M, Okada E, Toyama Y, Iwanami A, Ishii K, Ogata K, Asahara H, Matsumoto N, Nakamura M, Matsumoto M, Ikegawa S. Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst PLoS One. 2015.11; ( PubMed, DOI )

Ryosuke Tateishi , Nobuko Akiyama , Maki Miyauchi, Riko Yoshinaga, Hiroki Sasanuma, Takashi Kudo, Miki Shimbo, Masahiro Shinohara, Koji Obata, Jun-ichiro Inoue, Masaki Shirakawa, Dai Shiba, Hiroshi Asahara, Nobuaki Yoshida, Satoru Takahashi, Hironobu Morita , Taishin Akiyama. Hypergravity Provokes a Temporary Reduction in CD4+CD8+ Thymocyte Number and a Persistent Decrease in Medullary Thymic Epithelial Cell Frequency in Mice. PLoS One. 2015.10; 10 ( PubMed, DOI )

Gernapudi R, Wolfson B, Zhang Y, Yao Y, Yang P, Asahara H, Zhou Q. MicroRNA 140 Promotes Expression of Long Noncoding RNA NEAT1 in Adipogenesis Mol Cell Biol. 2015.10; ( PubMed, DOI )

Matsubara Y, Kato T, Kashimada K, Tanaka H, Zhi Z, Ichinose S, Mizutani S, Morio T, Chiba T, Ito Y, Saga Y, Takada S, Asahara H. TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis. Stem Cells Dev. 2015.05; 1164-1170. ( PubMed, DOI )

Otabe Koji, Nakahara Hiroyuki, Hasegawa Akihiko, Matsukawa Tetsuya, Ayabe Fumiaki, Onizuka Naoko, Inui Masafumi, Takada Shuji, Ito Yoshiaki, Sekiya Ichiro, Muneta Takeshi, Lotz Martin, Asahara Hiroshi. Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo. J Orthop Res. 2015.01; 33 (1): 1-8. ( PubMed, DOI )

Nagata Eiko, Kano Hiroki, Kato Fumiko, Yamaguchi Rie, Nakashima Shinichi, Takayama Shinichiro, Kosaki Rika, Tonoki Hidefumi, Mizuno Seiji, Watanabe Satoshi, Yoshiura Koh-Ichiro, Kosho Tomoki, Hasegawa Tomonobu, Kimizuka Mamori, Suzuki Atsushi, Shimizu Kenji, Ohashi Hirofumi, Haga Nobuhiko, Numabe Hironao, Horii Emiko, Nagai Toshiro, Yoshihashi Hiroshi, Nishimura Gen, Toda Tatsushi, Takada Shuji, Yokoyama Shigetoshi, Asahara Hiroshi, Sano Shinichiro, Fukami Maki, Ikegawa Shiro, Ogata Tsutomu. Japanese founder duplications/triplications involving BHLHA9 are associated with split-hand/foot malformation with or without long bone deficiency and Gollop-Wolfgang complex. Orphanet J Rare Dis. 2014.10; 9 (1): 125. ( PubMed, DOI )

Miyata Kohei, Yotsumoto Fusanori, Nam Sung Ouk, Odawara Takashi, Manabe Sadao, Ishikawa Toyokazu, Itamochi Hiroaki, Kigawa Junzo, Takada Shuji, Asahara Hiroshi, Kuroki Masahide, Miyamoto Shingo. Contribution of transcription factor, SP1, to the promotion of HB-EGF expression in defense mechanism against the treatment of irinotecan in ovarian clear cell carcinoma. Cancer Med. 2014.10; 3 (5): 1159-1169. ( PubMed, DOI )

Miyata Kohei, Miyata Tomoko, Nakabayashi Kazuhiko, Okamura Kohji, Naito Masashi, Kawai Tomoko, Takada Shuji, Kato Kiyoko, Miyamoto Shingo, Hata Kenichiro, Asahara Hiroshi. DNA methylation analysis of human myoblasts during in vitro myogenic differentiation: de novo methylation of promoters of muscle-related genes and its involvement in transcriptional down-regulation. Hum Mol Genet. 2014.09; 24 (2): 410-423. ( PubMed, DOI )

Takasawa Kei, Kashimada Kenichi, Pelosi Emanuele, Takagi Masatoshi, Morio Tomohiro, Asahara Hiroshi, Schlessinger David, Mizutani Shuki, Koopman Peter. FOXL2 transcriptionally represses Sf1 expression by antagonizing WT1 during ovarian development in mice. FASEB J. 2014.05; 28 (5): 2020-2028. ( PubMed, DOI )

Shinohara Masahiro, Chang Betty Y, Buggy Joseph J, Nagai Yusuke, Kodama Tatsuhiko, Asahara Hiroshi, Takayanagi Hiroshi. The orally available Btk inhibitor ibrutinib (PCI-32765) protects against osteoclast-mediated bone loss. Bone. 2014.03; 60 8-15. ( PubMed, DOI )

Onizuka Naoko, Ito Yoshiaki, Inagawa Masayo, Nakahara Hiroyuki, Takada Shuji, Lotz Martin, Toyama Yoshiaki, Asahara Hiroshi. The Mohawk homeobox transcription factor regulates the differentiation of tendons and volar plates. J Orthop Sci. 2014.01; 19 (1): 172-180. ( PubMed, DOI )

Akasaki Y, Hasegawa A, Saito M, Asahara H, Iwamoto Y, Lotz M K. Dysregulated FOXO transcription factors in articular cartilage in aging and osteoarthritis. Osteoarthritis Cartilage. 2014.01; 22 (1): 162-170. ( PubMed, DOI )

Matsubara Yohei, Chiba Tomoki, Kashimada Kenichi, Morio Tomohiro, Takada Shuji, Mizutani Shuki, Asahara Hiroshi. Transcription activator-like effector nuclease-mediated transduction of exogenous gene into IL2RG locus. Sci Rep. 2014; 4 5043. ( PubMed, DOI )

Inui Masafumi, Miyado Mami, Igarashi Maki, Tamano Moe, Kubo Atsushi, Yamashita Satoshi, Asahara Hiroshi, Fukami Maki, Takada Shuji. Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system. Sci Rep. 2014; 4 5396. ( PubMed, DOI )

Yoshitaka Teruhito, Kawai Akira, Miyaki Shigeru, Numoto Kunihiko, Kikuta Kazutaka, Ozaki Toshifumi, Lotz Martin, Asahara Hiroshi. Analysis of microRNAs expressions in chondrosarcoma. J Orthop Res. 2013.12; 31 (12): 1992-1998. ( PubMed, DOI )

Kato Tomoko, Miyata Kohei, Sonobe Miku, Yamashita Satoshi, Tamano Moe, Miura Kento, Kanai Yoshiakira, Miyamoto Shingo, Sakuma Tetsushi, Yamamoto Takashi, Inui Masafumi, Kikusui Takefumi, Asahara Hiroshi, Takada Shuji. Production of Sry knockout mouse using TALEN via oocyte injection. Sci Rep. 2013.11; 3 3136. ( PubMed, DOI )

Shimizu Hirohito, Kubo Atsushi, Uchibe Kenta, Hashimoto Megumi, Yokoyama Shigetoshi, Takada Shuji, Mitsuoka Kazuhiko, Asahara Hiroshi. The AERO system: a 3D-like approach for recording gene expression patterns in the whole mouse embryo. PLoS One. 2013.10; 8 (10): e75754. ( PubMed, DOI )

Takada Shuji, Sato Tempei, Ito Yoshiaki, Yamashita Satoshi, Kato Tomoko, Kawasumi Miyuri, Kanai-Azuma Masami, Igarashi Arisa, Kato Tomomi, Tamano Moe, Asahara Hiroshi. Targeted gene deletion of miRNAs in mice by TALEN system. PLoS One. 2013.10; 8 (10): e76004. ( PubMed, DOI )

Gibson Gary, Asahara Hiroshi. microRNAs and cartilage. J Orthop Res. 2013.09; 31 (9): 1333-1344. ( PubMed, DOI )

Nakahara Hiroyuki, Hasegawa Akihiko, Otabe Koji, Ayabe Fumiaki, Matsukawa Tetsuya, Onizuka Naoko, Ito Yoshiaki, Ozaki Toshifumi, Lotz Martin K, Asahara Hiroshi. Transcription factor Mohawk and the pathogenesis of human anterior cruciate ligament degradation. Arthritis Rheum. 2013.08; 65 (8): 2081-2089. ( PubMed, DOI )

Rakoczy Joanna, Fernandez-Valverde Selene L, Glazov Evgeny A, Wainwright Elanor N, Sato Tempei, Takada Shuji, Combes Alexander N, Korbie Darren J, Miller David, Grimmond Sean M, Little Melissa H, Asahara Hiroshi, Mattick John S, Taft Ryan J, Wilhelm Dagmar. MicroRNAs-140-5p/140-3p modulate Leydig cell numbers in the developing mouse testis. Biol Reprod. 2013.06; 88 (6): 143. ( PubMed, DOI )

Hasegawa Akihiko, Nakahara Hiroyuki, Kinoshita Mitsuo, Asahara Hiroshi, Koziol James, Lotz Martin K. Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. Arthritis Res Ther. 2013.02; 15 (1): R29. ( PubMed, DOI )

Matsukawa Tetsuya, Sakai Tadahiro, Yonezawa Tomo, Hiraiwa Hideki, Hamada Takashi, Nakashima Motoshige, Ono Yohei, Ishizuka Shinya, Nakahara Hiroyuki, Lotz Martin K, Asahara Hiroshi, Ishiguro Naoki. MicroRNA-125b regulates the expression of aggrecanase-1 (ADAMTS-4) in human osteoarthritic chondrocytes. Arthritis Res Ther. 2013.02; 15 (1): R28. ( PubMed, DOI )

Takata Akemi, Otsuka Motoyuki, Yoshikawa Takeshi, Kishikawa Takahiro, Hikiba Yohko, Obi Shuntaro, Goto Tadashi, Kang Young Jun, Maeda Shin, Yoshida Haruhiko, Omata Masao, Asahara Hiroshi, Koike Kazuhiko. MicroRNA-140 acts as a liver tumor suppressor by controlling NF-kappaB activity by directly targeting DNA methyltransferase 1 (Dnmt1) expression. Hepatology. 2013.01; 57 (1): 162-170. ( PubMed, DOI )

Watanabe Takashi, Oyama Toshinao, Asada Maki, Harada Daisuke, Ito Yoshiaki, Inagawa Masayo, Suzuki Yutaka, Sugano Sumio, Katsube Ken-ichi, Karsenty Gerard, Komori Toshihisa, Kitagawa Motoo, Asahara Hiroshi. MAML1 enhances the transcriptional activity of Runx2 and plays a role in bone development. PLoS Genet. 2013.01; 9 (1): e1003132. ( PubMed, DOI )

Furumatsu Takayuki, Asahara Hiroshi. Chromatin assembly and in vitro transcription analyses for evaluation of individual protein activities in multicomponent transcriptional complexes. Methods Mol Biol. 2013; 977 193-202. ( PubMed, DOI )

Miyaki Shigeru, Asahara Hiroshi. Macro view of microRNA function in osteoarthritis. Nat Rev Rheumatol. 2012.09; 8 (9): 543-552. ( PubMed, DOI )

Ito Yoshiaki, Kayama Tomohiro, Asahara Hiroshi. A systems approach and skeletal myogenesis. Comp Funct Genomics. 2012.09; 2012 759407. ( PubMed, DOI )

Shuji Takada, Hiroshi Asahara. Current strategies for microRNA research. Mod Rheumatol. 2012.09; 22 (5): 645-653. ( PubMed, DOI )

Fukami Maki, Tsuchiya Takayoshi, Takada Shuji, Kanbara Akiko, Asahara Hiroshi, Igarashi Arisa, Kamiyama Yasunori, Nishimura Gen, Ogata Tsutomu. Complex genomic rearrangement in the SOX9 5' region in a patient with Pierre Robin sequence and hypoplastic left scapula. Am J Med Genet A. 2012.07; 158A (7): 1529-1534. ( PubMed, DOI )

Uchibe Kenta, Shimizu Hirohito, Yokoyama Shigetoshi, Kuboki Takuo, Asahara Hiroshi. Identification of novel transcription-regulating genes expressed during murine molar development. Dev Dyn. 2012.07; 241 (7): 1217-1226. ( PubMed, DOI )

Yamashita Satoshi, Miyaki Shigeru, Kato Yoshio, Yokoyama Shigetoshi, Sato Tempei, Barrionuevo Francisco, Akiyama Haruhiko, Scherer Gerd, Takada Shuji, Asahara Hiroshi. L-Sox5 and Sox6 proteins enhance chondrogenic miR-140 microRNA expression by strengthening dimeric Sox9 activity. J Biol Chem. 2012.06; 287 (26): 22206-22215. ( PubMed, DOI )

Elaine F Chan, Ricky Harjanto, Hiroshi Asahara, Nozomu Inoue, Koichi Masuda, William D Bugbee, Gary S Firestein, Harish S Hosalkar, Martin K Lotz, Robert L Sah. Structural and functional maturation of distal femoral cartilage and bone during postnatal development and growth in humans and mice. Orthop. Clin. North Am.. 2012.04; 43 (2): 173-85, v. ( PubMed, DOI )

Satoshi Yamashita and Hiroshi Asahara. miRNA Functions in Arthritis Current Rheumatology Reviews. 2012; 8 98-102.

Shogo Wada, Yoshio Kato, Mitsuharu Okutsu, Shigeru Miyaki, Katsuhiko Suzuki, Zhen Yan, Stefano Schiaffino, Hiroshi Asahara, Takashi Ushida, Takayuki Akimoto. Translational suppression of atrophic regulators by microRNA-23a integrates resistance to skeletal muscle atrophy. J. Biol. Chem.. 2011.11; 286 (44): 38456-38465. ( PubMed, DOI )

Hiroshi Asahara. Switching gears to an arthritis gene expression network by SirT1 cleavage. Arthritis Rheum.. 2011.08; 63 (8): 2161-2163. ( PubMed, DOI )

Shigetoshi Yokoyama, Hiroshi Asahara. The myogenic transcriptional network. Cell. Mol. Life Sci.. 2011.06; 68 (11): 1843-1849. ( PubMed, DOI )

Motoharu Awazawa, Kohjiro Ueki, Kazunori Inabe, Toshimasa Yamauchi, Naoto Kubota, Kazuma Kaneko, Masatoshi Kobayashi, Aya Iwane, Takayoshi Sasako, Yukiko Okazaki, Mitsuru Ohsugi, Iseki Takamoto, Satoshi Yamashita, Hiroshi Asahara, Shizuo Akira, Masato Kasuga, Takashi Kadowaki. Adiponectin enhances insulin sensitivity by increasing hepatic IRS-2 expression via a macrophage-derived IL-6-dependent pathway. Cell Metab.. 2011.04; 13 (4): 401-412. ( PubMed, DOI )

Maki Asada, Alexander Rauch, Hirohito Shimizu, Hiromi Maruyama, Shigeru Miyaki, Masafumi Shibamori, Hideki Kawasome, Hironobu Ishiyama, Jan Tuckermann, Hiroshi Asahara. DNA binding-dependent glucocorticoid receptor activity promotes adipogenesis via Krüppel-like factor 15 gene expression. Lab. Invest.. 2011.02; 91 (2): 203-215. ( PubMed, DOI )

Reiko Mizutani, Kazuaki Nakamura, Shigetoshi Yokoyama, Atsushi Sanbe, Shinji Kusakawa, Yuki Miyamoto, Tomohiro Torii, Hiroshi Asahara, Haruo Okado, Junji Yamauchi, Akito Tanoue. Developmental expression of sorting nexin 3 in the mouse central nervous system. Gene Expr. Patterns. 2011.01; 11 (1-2): 33-40. ( PubMed, DOI )

Takayuki Furumatsu, Hiroshi Asahara. Histone acetylation influences the activity of Sox9-related transcriptional complex. Acta Med. Okayama. 2010.12; 64 (6): 351-357. ( PubMed, DOI )

Miyaki S, Sato T, Inoue A, Otsuki S, Ito Y, Yokoyama S, Kato Y, Takemoto F, Nakasa T, Yamashita S, Takada S, Lotz MK, Ueno-Kudo H, Asahara H. MicroRNA-140 plays dual roles in both cartilage development and homeostasis. Genes Dev. 2010.06; 24 (11): 1173-1185. ( PubMed, DOI )

Ito Y, Toriuchi N, Yoshitaka T, Ueno-Kudoh H, Sato T, Yokoyama S, Nishida K, Akimoto T, Takahashi M, Miyaki S, Asahara H. The Mohawk homeobox gene is a critical regulator of tendon differentiation. Proc Natl Acad Sci USA. 2010.06; 107 (23): 10538-10542. ( PubMed, DOI )

Shuhei Otsuki, Sarah R Hanson, Shigeru Miyaki, Shawn P Grogan, Mitsuo Kinoshita, Hiroshi Asahara, Chi-Huey Wong, Martin K Lotz. Extracellular sulfatases support cartilage homeostasis by regulating BMP and FGF signaling pathways. Proc. Natl. Acad. Sci. U.S.A.. 2010.06; 107 (22): 10202-10207. ( PubMed, DOI )

Nakanishi K, Nakasa T, Tanaka N, Ishikawa M, Yamada K, Yamasaki K, Kamei N, Izumi B, Adachi N, Miyaki S, Asahara H, Ochi M. . Responses of microRNAs 124a and 223 following spinal cord injury in mice. Spinal Cord. 2010.03; 48 (3): 192-196. ( PubMed, DOI )

Reiji Higashiyama, Shigeru Miyaki, Satoshi Yamashita, Teruhito Yoshitaka, Görel Lindman, Yoshiaki Ito, Takahisa Sasho, Kazuhisa Takahashi, Martin Lotz, Hiroshi Asahara. Correlation between MMP-13 and HDAC7 expression in human knee osteoarthritis. Mod Rheumatol. 2010.02; 20 (1): 11-17. ( PubMed, DOI )

Shuhei Otsuki, Shawn P Grogan, Shigeru Miyaki, Mitsuo Kinoshita, Hiroshi Asahara, Martin K Lotz. Tissue neogenesis and STRO-1 expression in immature and mature articular cartilage. J. Orthop. Res.. 2010.01; 28 (1): 96-102. ( PubMed, DOI )

Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H. A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Dev Cell. 2009.12; 17 (6): 836-848. ( PubMed, DOI )

Kato Y, Miyaki S, Yokoyama S, Omori S, Inoue A, Horiuchi M, Asahara H. Real-time functional imaging for monitoring miR-133 during myogenic differentiation. The international journal of biochemistry & cell biology. 2009.11; 41 (11): 2225-31. ( PubMed, DOI )

Abe Y, Oka A, Mizuguchi M, Igarashi T, Ishikawa S, Aburatani H, Yokoyama S, Asahara H, Nagao K, Yamada M, Miyashita T. EYA4, deleted in a case with middle interhemispheric variant of holoprosencephaly, interacts with SIX3 both physically and functionally. Human mutation. 2009.10; 30 (10): E946-55. ( PubMed, DOI )

Ito T, Yadav N, Lee J, Furumatsu T, Yamashita S, Yoshida K, Taniguchi N, Hashimoto M, Tsuchiya M, Ozaki T, Lotz M, Bedford MT, Asahara H. Arginine methyltransferase CARM1/PRMT4 regulates endochondral ossification. BMC developmental biology. 2009.09; 9 47. ( PubMed, DOI )

Miyaki S, Nakasa T, Otsuki S, Grogan SP, Higashiyama R, Inoue A, Kato Y, Sato T, Lotz MK, Asahara H. MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates interleukin-1 responses. Arthritis and rheumatism. 2009.09; 60 (9): 2723-30. ( PubMed, DOI )

Nagata Y, Nakasa T, Mochizuki Y, Ishikawa M, Miyaki S, Shibuya H, Yamasaki K, Adachi N, Asahara H, Ochi M. Induction of apoptosis in the synovium of mice with autoantibody-mediated arthritis by the intraarticular injection of double-stranded MicroRNA-15a. Arthritis and rheumatism. 2009.09; 60 (9): 2677-83. ( PubMed, DOI )

Yamashita S, Andoh M, Ueno-Kudoh H, Sato T, Miyaki S, Asahara H. Sox9 directly promotes Bapx1 gene expression to repress Runx2 in chondrocytes. Experimental cell research. 2009.08; 315 (13): 2231-40. ( PubMed, DOI )

Grogan SP, Miyaki S, Asahara H, D'Lima DD, Lotz MK. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis. Arthritis research & therapy. 2009.06; 11 (3): R85. ( PubMed, DOI )

Furumatsu T, Ozaki T, Asahara H. Smad3 activates the Sox9-dependent transcription on chromatin. The international journal of biochemistry & cell biology. 2009.05; 41 (5): 1198-204. ( PubMed, DOI )

FANTOM Consortium., Suzuki H, Forrest AR, van Nimwegen E, Daub CO, Balwierz PJ, Irvine KM, Lassmann T, Ravasi T, Hasegawa Y, de Hoon MJ, Katayama S, Schroder K, Carninci P, Tomaru Y, Kanamori-Katayama M, Kubosaki A, Akalin A, Ando Y, Arner E, Asada M, Asahara H, Bailey T, Bajic VB, Bauer D, Beckhouse AG, Bertin N, Björkegren J, Brombacher F, Bulger E, Chalk AM, Chiba J, Cloonan N, Dawe A, Dostie J, Engström PG, Essack M, Faulkner GJ, Fink JL, Fredman D, Fujimori K, Furuno M, Gojobori T, Gough J, Grimmond SM, Gustafsson M, Hashimoto M, Hashimoto T, Hatakeyama M, Heinzel S, Hide W, Hofmann O, Hörnquist M, Huminiecki L, Ikeo K, Imamoto N, Inoue S, Inoue Y, Ishihara R, Iwayanagi T, Jacobsen A, Kaur M, Kawaji H, Kerr MC, Kimura R, Kimura S, Kimura Y, Kitano H, Koga H, Kojima T, Kondo S, Konno T, Krogh A, Kruger A, Kumar A, Lenhard B, Lennartsson A, Lindow M, Lizio M, Macpherson C, Maeda N, Maher CA, Maqungo M, Mar J, Matigian NA, Matsuda H, Mattick JS, Meier S, Miyamoto S, Miyamoto-Sato E, Nakabayashi K, Nakachi Y, Nakano M, Nygaard S, Okayama T, Okazaki Y, Okuda-Yabukami H, Orlando V, Otomo J, Pachkov M, Petrovsky N, Plessy C, Quackenbush J, Radovanovic A, Rehli M, Saito R, Sandelin A, Schmeier S, Schönbach C, Schwartz AS, Semple CA, Sera M, Severin J, Shirahige K, Simons C, St Laurent G, Suzuki M, Suzuki T, Sweet MJ, Taft RJ, Takeda S, Takenaka Y, Tan K, Taylor MS, Teasdale RD, Tegnér J, Teichmann S, Valen E, Wahlestedt C, Waki K, Waterhouse A, Wells CA, Winther O, Wu L, Yamaguchi K, Yanagawa H, Yasuda J, Zavolan M, Hume DA, Riken Omics Science Center., Arakawa T, Fukuda S, Imamura K, Kai C, Kaiho A, Kawashima T, Kawazu C, Kitazume Y, Kojima M, Miura H, Murakami K, Murata M, Ninomiya N, Nishiyori H, Noma S, Ogawa C, Sano T, Simon C, Tagami M, Takahashi Y, Kawai J, Hayashizaki Y. The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line. Nature genetics. 2009.05; 41 (5): 553-62. ( PubMed, DOI )

Yamasaki K, Nakasa T, Miyaki S, Ishikawa M, Deie M, Adachi N, Yasunaga Y, Asahara H, Ochi M. Expression of MicroRNA-146a in osteoarthritis cartilage. Arthritis and rheumatism. 2009.04; 60 (4): 1035-41. ( PubMed, DOI )

Hikata T, Takaishi H, Takito J, Hakozaki A, Furukawa M, Uchikawa S, Kimura T, Okada Y, Matsumoto M, Yoshimura A, Nishimura R, Reddy SV, Asahara H, Toyama Y. PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts. Blood. 2009.03; 113 (10): 2202-12. ( PubMed, DOI )

Kumagishi K, Nishida K, Yamaai T, Momota R, Miyaki S, Hirohata S, Naito I, Asahara H, Ninomiya Y, Ohtsuka A. A disintegrin and metalloproteinase with thrombospondin motifs 9 (ADAMTS9) expression by chondrocytes during endochondral ossification. Archives of histology and cytology. 2009; 72 (3): 175-85. ( PubMed )

Shimizu H, Uchibe K, Asahara H. Large-scale whole mount in situ hybridization of mouse embryos. Methods in molecular biology (Clifton, N.J.). 2009; 577 167-79. ( PubMed, DOI )

Sakai N, Terami H, Suzuki S, Haga M, Nomoto K, Tsuchida N, Morohashi K, Saito N, Asada M, Hashimoto M, Harada D, Asahara H, Ishikawa T, Shimada F, Sakurada K. Identification of NR5A1 (SF-1/AD4BP) gene expression modulators by large-scale gain and loss of function studies. The Journal of endocrinology. 2008.09; 198 (3): 489-97. ( PubMed, DOI )

Nakasa T, Miyaki S, Okubo A, Hashimoto M, Nishida K, Ochi M, Asahara H. Expression of microRNA-146 in rheumatoid arthritis synovial tissue. Arthritis and rheumatism. 2008.05; 58 (5): 1284-92. ( PubMed, DOI )

Hashimoto M, Nakasa T, Hikata T, Asahara H. Molecular network of cartilage homeostasis and osteoarthritis. Medicinal research reviews. 2008.05; 28 (3): 464-81. ( PubMed, DOI )

Yokoyama S, Hashimoto M, Shimizu H, Ueno-Kudoh H, Uchibe K, Kimura I, Asahara H. Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken. Gene expression patterns : GEP. 2008.02; 8 (3): 155-60. ( PubMed, DOI )

Teruhito Yoshitaka, Hiroshi Asahara,. Epigenetics and Cartilage Regulation US Musculoskeletal Review. 2008; 3 (1): 45-47.

Shimizu H, Yokoyama S, Asahara H. Growth and differentiation of the developing limb bud from the perspective of chondrogenesis. Development, growth & differentiation. 2007.08; 49 (6): 449-54. ( PubMed, DOI )

Taniguchi N, Yoshida K, Ito T, Tsuda M, Mishima Y, Furumatsu T, Ronfani L, Abeyama K, Kawahara K, Komiya S, Maruyama I, Lotz M, Bianchi ME, Asahara H. Stage-specific secretion of HMGB1 in cartilage regulates endochondral ossification. Molecular and cellular biology. 2007.08; 27 (16): 5650-63. ( PubMed, DOI )

Sato K, Suematsu A, Nakashima T, Takemoto-Kimura S, Aoki K, Morishita Y, Asahara H, Ohya K, Yamaguchi A, Takai T, Kodama T, Chatila TA, Bito H, Takayanagi H. Regulation of osteoclast differentiation and function by the CaMK-CREB pathway. Nature medicine. 2006.12; 12 (12): 1410-6. ( PubMed, DOI )

Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H. Sox9 and p300 cooperatively regulate chromatin-mediated transcription. The Journal of biological chemistry. 2005.10; 280 (42): 35203-8. ( PubMed, DOI )

Furumatsu T, Tsuda M, Taniguchi N, Tajima Y, Asahara H. Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment. The Journal of biological chemistry. 2005.03; 280 (9): 8343-50. ( PubMed, DOI )

Kawakami Y, Tsuda M, Takahashi S, Taniguchi N, Esteban CR, Zemmyo M, Furumatsu T, Lotz M, Izpisúa Belmonte JC, Asahara H. Transcriptional coactivator PGC-1alpha regulates chondrogenesis via association with Sox9. Proceedings of the National Academy of Sciences of the United States of America. 2005.02; 102 (7): 2414-9. ( PubMed, DOI )

Omoto S, Nishida K, Yamaai Y, Shibahara M, Nishida T, Doi T, Asahara H, Nakanishi T, Inoue H, Takigawa M. Expression and localization of connective tissue growth factor (CTGF/Hcs24/CCN2) in osteoarthritic cartilage. Osteoarthritis and cartilage. 2004.10; 12 (10): 771-8. ( PubMed, DOI )

Kato T, Asahara H, Kurokawa MS, Fujisawa K, Hasunuma T, Inoue H, Tsuda M, Takahashi S, Motokawa S, Sumida T, Nishioka K. HTLV-I env protein acts as a major antigen in patients with HTLV-I-associated arthropathy. Clinical rheumatology. 2004.10; 23 (5): 400-9. ( PubMed, DOI )

Nishida K, Komiyama T, Miyazawa S, Shen ZN, Furumatsu T, Doi H, Yoshida A, Yamana J, Yamamura M, Ninomiya Y, Inoue H, Asahara H. Histone deacetylase inhibitor suppression of autoantibody-mediated arthritis in mice via regulation of p16INK4a and p21(WAF1/Cip1) expression. Arthritis and rheumatism. 2004.10; 50 (10): 3365-76. ( PubMed, DOI )

Kawakami Y, Rodríguez-León J, Koth CM, Büscher D, Itoh T, Raya A, Ng JK, Esteban CR, Takahashi S, Henrique D, Schwarz MF, Asahara H, Izpisúa Belmonte JC. MKP3 mediates the cellular response to FGF8 signalling in the vertebrate limb. Nature cell biology. 2003.06; 5 (6): 513-9. ( PubMed, DOI )

Canettieri G, Morantte I, Guzmán E, Asahara H, Herzig S, Anderson SD, Yates JR 3rd, Montminy M. Attenuation of a phosphorylation-dependent activator by an HDAC-PP1 complex. Nature structural biology. 2003.03; 10 (3): 175-81. ( PubMed, DOI )

Asahara H, Tartare-Deckert S, Nakagawa T, Ikehara T, Hirose F, Hunter T, Ito T, Montminy M. Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with nucleosome assembly protein 1 in vitro. Molecular and cellular biology. 2002.05; 22 (9): 2974-83. ( PubMed )

W Xu, H Chen, K Du, H Asahara, M Tini, B M Emerson, M Montminy, R M Evans. A transcriptional switch mediated by cofactor methylation. Science. 2001.12; 294 (5551): 2507-2511. ( PubMed, DOI )

H Asahara, B Santoso, E Guzman, K Du, P A Cole, I Davidson, M Montminy. Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB. Mol. Cell. Biol.. 2001.12; 21 (23): 7892-7900. ( PubMed, DOI )

F Long, E Schipani, H Asahara, H Kronenberg, M Montminy. The CREB family of activators is required for endochondral bone development. Development. 2001.02; 128 (4): 541-550. ( PubMed )

T Sekine, K Masuko-Hongo, T Matsui, H Asahara, M Takigawa, K Nishioka, T Kato. Recognition of YKL-39, a human cartilage related protein, as a target antigen in patients with rheumatoid arthritis. Ann. Rheum. Dis.. 2001.01; 60 (1): 49-54. ( PubMed )

K Asaumi, T Nakanishi, H Asahara, H Inoue, M Takigawa. Expression of neurotrophins and their receptors (TRK) during fracture healing. Bone. 2000.06; 26 (6): 625-633. ( PubMed )

K Du, H Asahara, U S Jhala, B L Wagner, M Montminy. Characterization of a CREB gain-of-function mutant with constitutive transcriptional activity in vivo. Mol. Cell. Biol.. 2000.06; 20 (12): 4320-4327. ( PubMed )

M Shibahara, K Nishida, H Asahara, T Yoshikawa, S Mitani, Y Kondo, H Inoue. Increased osteocyte apoptosis during the development of femoral head osteonecrosis in spontaneously hypertensive rats. Acta Med. Okayama. 2000.04; 54 (2): 67-74. ( PubMed, DOI )

L F Michael, H Asahara, A I Shulman, W L Kraus, M Montminy. The phosphorylation status of a cyclic AMP-responsive activator is modulated via a chromatin-dependent mechanism. Mol. Cell. Biol.. 2000.03; 20 (5): 1596-1603. ( PubMed )

H Asahara, S Dutta, H Y Kao, R M Evans, M Montminy. Pbx-Hox heterodimers recruit coactivator-corepressor complexes in an isoform-specific manner. Mol. Cell. Biol.. 1999.12; 19 (12): 8219-8225. ( PubMed )

M Suzuki, H Asahara, S Endo, K Inada, M Doi, K Kuroda, A Ogawa. Increased levels of nitrite/nitrate in the cerebrospinal fluid of patients with subarachnoid hemorrhage. Neurosurg Rev. 1999.10; 22 (2-3): 96-98. ( PubMed )

T Maeda, H Keino, H Asahara, M Taniguchi, K Nishioka, T Sumida. Decreased TCR AV24AJ18+ double-negative T cells in rheumatoid synovium. Rheumatology (Oxford). 1999.02; 38 (2): 186-188. ( PubMed )

K Okamoto, H Asahara, T Kobayashi, H Matsuno, T Hasunuma, T Kobata, T Sumida, K Nishioka. Induction of apoptosis in the rheumatoid synovium by Fas ligand gene transfer. Gene Ther.. 1998.03; 5 (3): 331-338. ( PubMed, DOI )

T Sumida, T Hasunuma, H Asahara, T Maeda, K Nishioka. Rheumatoid arthritis and apoptosis. Intern. Med.. 1998.02; 37 (2): 184-188. ( PubMed )

H Takagoshi, H Hashizume, K Nishida, S Masaoka, H Asahara, H Inoue. Fibrous structure and connection surrounding the metacarpophalangeal joint. Acta Med. Okayama. 1998.02; 52 (1): 19-26. ( PubMed, DOI )

J Pu, K Nishida, H Inoue, H Asahara, A Ohtsuka, T Murakami. Mast cells in osteoarthritic and rheumatoid arthritic synovial tissues of the human knee. Acta Med. Okayama. 1998.02; 52 (1): 35-39. ( PubMed, DOI )

K Fujisawa, K Okamoto, H Asahara, T Hasunuma, T Kobata, T Kato, T Sumida, K Nishioka. Evidence for autoantigens of Env/Tax proteins in human T cell leukemia virus type I Env-pX transgenic mice. Arthritis Rheum.. 1998.01; 41 (1): 101-109. ( PubMed, DOI )

T Kobata, K Takasaki, H Asahara, N M Hong, K Masuko-Hongo, T Kato, S Hirose, T Shirai, N Kayagaki, H Yagita, K Okumura, K Nishioka. Apoptosis with FasL+ cell infiltration in the periphery and thymus of corrected autoimmune mice. Immunology. 1997.10; 92 (2): 206-213. ( PubMed )

C Xue, T Hasunuma, H Asahara, W Yin, T Maeda, K Fujisawa, Y Dong, T Sumida, K Nishioka. Transcriptional regulation of the HOX4C gene by basic fibroblast growth factor on rheumatoid synovial fibroblasts. Arthritis Rheum.. 1997.09; 40 (9): 1628-1635. ( PubMed, DOI )

H Asahara, K Fujisawa, T Kobata, T Hasunuma, T Maeda, M Asanuma, N Ogawa, H Inoue, T Sumida, K Nishioka. Direct evidence of high DNA binding activity of transcription factor AP-1 in rheumatoid arthritis synovium. Arthritis Rheum.. 1997.05; 40 (5): 912-918. ( PubMed, DOI )

M Suzuki, A Ogawa, H Asahara, S Endo, K Inada. Nitric oxide and vasospasm. J. Neurosurg.. 1997.04; 86 (4): 741-742. ( PubMed )

H Asahara, T Hasunuma, T Kobata, H Inoue, U Muller-Ladner, S Gay, T Sumida, K Nishioka. In situ expression of protooncogenes and Fas/Fas ligand in rheumatoid arthritis synovium. J. Rheumatol.. 1997.03; 24 (3): 430-435. ( PubMed )

T Sumida, T T Hoa, H Asahara, T Hasunuma, K Nishioka. T cell receptor of Fas-sensitive T cells in rheumatoid synovium. J. Immunol.. 1997.02; 158 (4): 1965-1970. ( PubMed )

T Hasunuma, N Kayagaki, H Asahara, S Motokawa, T Kobata, H Yagita, H Aono, T Sumida, K Okumura, K Nishioka. Accumulation of soluble Fas in inflamed joints of patients with rheumatoid arthritis. Arthritis Rheum.. 1997.01; 40 (1): 80-86. ( PubMed )

Yokoi I, Kabuto H, Habu H, Inada K, Toma J, Asahara H, Mori A.. Structure-activity relationships of guanidine compounds on brain nitric oxide synthase activity. Guanidino Compounds: 2,. 1997; 3-7.

Habu H, Yokoi I, Kabuto H, Asahara H, Toma J, Inada K, Mori A.. The role of nitric oxide on α-guanidinoglutaric acid induced epileptic discharges. Guanidino Compounds: 2,. 1997; 373-377.

H Asahara, T Hasumuna, T Kobata, H Yagita, K Okumura, H Inoue, S Gay, T Sumida, K Nishioka. Expression of Fas antigen and Fas ligand in the rheumatoid synovial tissue. Clin. Immunol. Immunopathol.. 1996.10; 81 (1): 27-34. ( PubMed )

T Hasunuma, T T Hoa, H Aono, H Asahara, S Yonehara, K Yamamoto, T Sumida, S Gay, K Nishioka. Induction of Fas-dependent apoptosis in synovial infiltrating cells in rheumatoid arthritis. Int. Immunol.. 1996.10; 8 (10): 1595-1602. ( PubMed )

K Fujisawa, H Asahara, K Okamoto, H Aono, T Hasunuma, T Kobata, Y Iwakura, S Yonehara, T Sumida, K Nishioka. Therapeutic effect of the anti-Fas antibody on arthritis in HTLV-1 tax transgenic mice. J. Clin. Invest.. 1996.07; 98 (2): 271-278. ( PubMed, DOI )

H Hashizume, H Asahara, K Nishida, H Inoue, T Konishiike. Histopathology of Kienböck's disease. Correlation with magnetic resonance and other imaging techniques. J Hand Surg Br. 1996.02; 21 (1): 89-93. ( PubMed )

H Asahara, A Kawai, Y Harada, M Senda, H Inoue. Spinal schwannomas: a review of 42 cases. Acta Med. Okayama. 1996.02; 50 (1): 25-28. ( PubMed, DOI )

H Asahara, I Yokoi, T Tamada, H Kabuto, N Ogawab, A Mori, H Inoue. Increased cerebrospinal fluid nitrite and nitrate levels in patients with lumbar spondylosis. Res. Commun. Mol. Pathol. Pharmacol.. 1996.01; 91 (1): 77-83. ( PubMed )

H Asahara, M Asanuma, N Ogawa, S Nishibayashi, H Inoue. High DNA-binding activity of transcription factor NF-kappa B in synovial membranes of patients with rheumatoid arthritis. Biochem. Mol. Biol. Int.. 1995.11; 37 (5): 827-832. ( PubMed )

H Asahara, H Kabuto, I Yokoi, H Habu, A Mori, H Inoue. Nitric oxide synthase inhibited by audouine in the rat brain. Neuroreport. 1995.05; 6 (8): 1146-1148. ( PubMed )

H Kabuto, I Yokoi, H Habu, H Asahara, A Mori. Inhibitory effect of arcaine on nitric oxide synthase in the rat brain. Neuroreport. 1995.02; 6 (3): 554-556. ( PubMed )

Hiroshi Asahara. Mechano-signal on tendon and ligament homeostasis via Mkx. International Symposium on AMED “Mechanobiology” Project 2020.02.08

Hiroshi Asahara. Mechano-signal Pathway Regulating Tendon and Ligament Via Mkx. AMED革新的先端研究開発支援事業（AMED-CREST,PRIME）「メカノバイオ」領域　令和元年度　領域会議 2020.02.06

Hiroshi Asahara. Homeostasis of tendons and ligaments connecting musculoskeletal system. Japan Bone Academy2019 2019.12.15

Hiroshi Asahara. Characterization of a novel Sox9 enhancer and down-stream targets with implications in Arthritis parthenogenesis and therapeutics.. SOX meeting2019 2019.10.01

淺原弘嗣. Lecture I“Cartilage homeostasis and arthritis pathogenesis via RNA regulation”. 16th Meeting of Bone Biology Forum 2019.08.16

浅原弘嗣 、茂久田翔、伊藤義晃、中道亮、杉山英二 、松崎時夫. Wwp2 maintains cartilage homeostasis through regulation of Adamts5. 第5回日本骨免疫学会 2019.06.26

Hiroshi Asahara, Sho Mokuda, Yoshiaki Ito, Masafumi Inui, Ryo Nakamichi, Tomoki Chiba, Ryota Kurimoto, Takahide Matsushima. miRNAs in arthritis pathogenesis and therapy. The 24th Annual Meeting of the RNA Society 2019.06.14

Hiroshi Asahara. miRNAs in arthritis pathogenesis and therapy. 2019 Keystone Symposia Conference 2019.04.17

淺原弘嗣. 腱・靭帯のメカノプロパティを応用した再生医療. FAOPS2019 2019.03.31

Hiroshi Asahara. Genome dynamics for chondrogenesis and miRNAs for cartilage homeostasis via Sox9. Cartilage GRC 2019 2019.03.21

Hiroshi Asahara. miRNAs in arthritis pathogenesis and therapy. The 20th Takeda Science Foundation Symposium on BioSciences 2019.02.01

Hiroshi Asahara. Mechano-signal pathway regulating tendon and ligament via Mkx. ORS Tendon Section 2018 Conference 2018.11.16

Hiroshi Asahara. miRNAs in arthritis pathogenesis and therapy. JAJ　RNA　2018 2018.11.05

Hiroshi Asahara. Transcriptional and translational regulation of musculoskeletal development and arthritis pathogenesis.. Marc Montminy's 62nd Birthday Symposium 2018.08.31

Hiroshi Asahara. The completely automated ChIP system performed by LabDroid ”Maholo”. Robotics and Semantic Systems for Biology (RSSB) 2 2018.01.27

Hiroshi Asahara. Transcription Factor Mkx Regulates Tendon Development, Homeostasis and Regeneration. Collagen Gordon Research Conference 2017.07.16 New London

Hiroshi Asahara. miRNA in Cancer, Arthritis and Homeostasis. The 43rd Naito Conference 2017.06.29

Hiroshi Asahara. Identification of targets of tumor suppressor microRNA-34a using a reporter library system . RNA　2017－Prague 2017.05.25 Prague

Hiroshi Asahara. Tendon development and regeneration analyzed by Mkx KO Rat. The 291st IMEG Seminar 2017.02.24

Hiroshi Asahara、Yoshiaki Ito. The role of microRNA-455 in cartilage. 第19回武田科学振興財団生命科学シンポジウム 2017.01.10

Hiroshi Asahara. Gene targeting of the transcription factor Mohawk in rats causes heterotopic ossification of Achilles tendon via failed tenogenesis. Stem Cell, Growth Factor Signaling and Bone Biology 2016.07.25 Hangzhou

Hiroshi Asahara,Tomoki Chiba,Kentaro Abe,Yoshiaki Ito. Inflammatory signal regulation at RNA level. Biochemistry and Molecular Biology 2015.12.01 Hyogo

浅原弘嗣. Skeletal development regulated at the level of RNA. 第12回Bone Biology Forum 2015.08.22 千葉

Hiroshi Asahara. Regulation of Hox-code at RNA level during skeletal development.. The ICMRS Conference and Xiangya Osteoporsis Forum 2015.04.10 China

Hiroshi Asahara. The Role of Mkx in Tendon and Spinal Disc Homeostasis and Regeneration. The ICMRS Conference and Xiangya Osteoporsis Forum 2015.04.06 China

Asahara H,Tempei Sato, Masafumi Inui. The Critical role of miRNAs at the edge of Hox-code during skeletal development. Gordon Research Conference 2015.03.23 Houston

Hiroshi Asahara. Genome editing technology for arthritis pathophysiology. INTERNATIONAL FORUM OF THE UNIVERSITY SCIENCE-2014 In conjunction with THE 2nd BIO RHEUMATOLOGY INTERNATIONAL CONGRESS(BRIC2014) THE 9th GLOBAL ARTHRITIS RESEARCH NETWORK(GRAN) 2014 MEETING MOSCOW 2014.06.06 Moscow