Nishita M., Nishikaku I., Yoshida E., Kamizaki K., Shibuya H., Matsumoto K., Minami Y.. Roil promotes invasion of lung adenocarcinoma cells through small GTPase Rif-mediated filopodia formation MOLECULAR BIOLOGY OF THE CELL. 2018.12; 29 (26): 231.

Sato A, Shibuya H.. Glycogen synthase kinase 3ß functions as a positive effector in the WNK signaling pathway. PLoS One. 2018.03; 13 e0193204.

Toshiyasu Goto, Junhei Matsuzawa, Shun-Ichiro Iemura, Tohru Natsume, Hiroshi Shibuya. WDR26 is a new partner of Axin1 in the canonical Wnt signaling pathway. FEBS Lett.. 2016.05; 590 (9): 1291-1303. ( PubMed, DOI )

Takashi Fukuzono, Strahil Iv Pastuhov, Okinobu Fukushima, Chun Li, Ayuna Hattori, Shun-Ichiro Iemura, Tohru Natsume, Hiroshi Shibuya, Hiroshi Hanafusa, Kunihiro Matsumoto, Naoki Hisamoto. Chaperone complex BAG2-HSC70 regulates localization of Caenorhabditis elegans leucine-rich repeat kinase LRK-1 to the Golgi. Genes Cells. 2016.04; 21 (4): 311-324. ( PubMed, DOI )

Isao Kii, Yuto Sumida, Toshiyasu Goto, Rie Sonamoto, Yukiko Okuno, Suguru Yoshida, Tomoe Kato-Sumida, Yuka Koike, Minako Abe, Yosuke Nonaka, Teikichi Ikura, Nobutoshi Ito, Hiroshi Shibuya, Takamitsu Hosoya, Masatoshi Hagiwara. Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun. 2016; 7 11391. ( PubMed, DOI )

Nobuyuki Shimizu, Shizuka Ishitani, Atsushi Sato, Hiroshi Shibuya, Tohru Ishitani. Hipk2 and PP1c cooperate to maintain Dvl protein levels required for Wnt signal transduction. Cell Rep. 2014.09; 8 (5): 1391-1404. ( PubMed, DOI )

Toshiyasu Goto, Atsushi Sato, Shungo Adachi, Shun-ichiro Iemura, Tohru Natsume, Hiroshi Shibuya. IQGAP1 protein regulates nuclear localization of β-catenin via importin-β5 protein in Wnt signaling. J. Biol. Chem.. 2013.12; 288 (51): 36351-36360. ( PubMed, DOI )

Masahiro Shimizu, Toshiyasu Goto, Atsushi Sato, Hiroshi Shibuya. WNK4 is an essential effector of anterior formation in FGF signaling. Genes Cells. 2013.06; 18 (6): 442-449. ( PubMed, DOI )

Atsushi Sato, Hiroshi Shibuya. WNK signaling is involved in neural development via Lhx8/Awh expression. PLoS ONE. 2013; 8 (1): e55301. ( PubMed, DOI )

Toshiyasu Goto, Atsushi Sato, Masahiro Shimizu, Shungo Adachi, Kiyotoshi Satoh, Shun-ichiro Iemura, Tohru Natsume, Hiroshi Shibuya. IQGAP1 functions as a modulator of dishevelled nuclear localization in Wnt signaling. PLoS ONE. 2013; 8 (4): e60865. ( PubMed, DOI )

Hiroshi Hanafusa, Kouki Ishikawa, Shin Kedashiro, Tsukasa Saigo, Shun-Ichiro Iemura, Tohru Natsume, Masayuki Komada, Hiroshi Shibuya, Atsuki Nara, Kunihiro Matsumoto. Leucine-rich repeat kinase LRRK1 regulates endosomal trafficking of the EGF receptor. Nat Commun. 2011.01; 2 158. ( PubMed, DOI )

Toshiyasu Goto, Akimasa Fukui, Hiroshi Shibuya, Ray Keller, Makoto Asashima. Xenopus furry contributes to release of microRNA gene silencing. Proc. Natl. Acad. Sci. U.S.A.. 2010.11; 107 (45): 19344-19349. ( PubMed, DOI )

Takeshi Maruyama, Hisae Kadowaki, Noriaki Okamoto, Atsushi Nagai, Isao Naguro, Atsushi Matsuzawa, Hiroshi Shibuya, Keiji Tanaka, Shigeo Murata, Kohsuke Takeda, Hideki Nishitoh, Hidenori Ichijo. CHIP-dependent termination of MEKK2 regulates temporal ERK activation required for proper hyperosmotic response. EMBO J.. 2010.08; 29 (15): 2501-2514. ( PubMed, DOI )

Akira Nifuji, Hisashi Ideno, Yoshio Ohyama, Rieko Takanabe, Ryoko Araki, Masumi Abe, Masaki Noda, Hiroshi Shibuya. Nemo-like kinase (NLK) expression in osteoblastic cells and suppression of osteoblastic differentiation. Exp. Cell Res.. 2010.04; 316 (7): 1127-1136. ( PubMed, DOI )

Eriko Ohnishi, Toshiyasu Goto, Atsushi Sato, Mi-Sun Kim, Shun-Ichiro Iemura, Tohru Ishitani, Tohru Natsume, Junji Ohnishi, Hiroshi Shibuya. Nemo-like kinase, an essential effector of anterior formation, functions downstream of p38 mitogen-activated protein kinase. Mol. Cell. Biol.. 2010.02; 30 (3): 675-683. ( PubMed, DOI )

Yukihide Watanabe, Susumu Itoh, Toshiyasu Goto, Eriko Ohnishi, Masako Inamitsu, Fumiko Itoh, Kiyotoshi Satoh, Eliza Wiercinska, Weiwen Yang, Liang Shi, Aya Tanaka, Naoko Nakano, A Mieke Mommaas, Hiroshi Shibuya, Peter Ten Dijke, Mitsuyasu Kato. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol. Cell. 2010.01; 37 (1): 123-134. ( PubMed, DOI )

Yasushi Ogawa, Yosuke Nonaka, Toshiyasu Goto, Eriko Ohnishi, Toshiyuki Hiramatsu, Isao Kii, Miyo Yoshida, Teikichi Ikura, Hiroshi Onogi, Hiroshi Shibuya, Takamitsu Hosoya, Nobutoshi Ito, Masatoshi Hagiwara. Development of a novel selective inhibitor of the Down syndrome-related kinase Dyrk1A. Nat Commun. 2010; 1 86. ( PubMed, DOI )

Hisamoto N, Moriguchi T, Urushiyama S, Mitani S, Shibuya H, Matsumoto K. Caenorhabditis elegans WNK-STE20 pathway regulates tube formation by modulating ClC channel activity. EMBO Rep. 2008.01; 9 (1): 70-75. ( PubMed, DOI )

Satoh K, Ohnishi J, Sato A, Takeyama M, Iemura S, Natsume T, Shibuya H. Nemo-like kinase-myocyte enhancer factor 2A signaling regulates anterior formation in Xenopus development. Mol Cell Biol. 2007.11; 27 (21): 7623-7630. ( PubMed, DOI )

Yang SS, Morimoto T, Rai T, Chiga M, Sohara E, Ohno M, Uchida K, Lin SH, Moriguchi T, Shibuya H, Kondo Y, Sasaki S, Uchida S. Molecular pathogenesis of pseudohypoaldosteronism type II: generation and analysis of a Wnk4(D561A/+) knockin mouse model. Cell Metabolism. 2007.05; 5 (5): 331-344. ( PubMed, DOI )

Matsubara Y, Kawasaki I, Urushiyama S, Yasuda T, Shirakata M, Iino Y, Shibuya H, Yamanashi Y. The adaptor-like protein ROG-1 is required for activation of the Ras-MAP kinase pathway and meiotic cell cycle progression in Caenorhabditis elegans. Genes Cells. 2007.03; 12 (3): 407-420. ( PubMed, DOI )

Yamada M, Ohnishi J, Ohkawara B, Iemura S, Satoh K, Hyodo-Miura J, Kawachi K, Natsume T, Shibuya H. NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF). J Biol Chem. 2006.07; 281 (30): 20749-20760. ( PubMed, DOI )

Honma M, Higuchi O, Shirakata M, Yasuda T, Shibuya H, Iemura S, Natsume T, Yamanashi Y. Dok-3 sequesters Grb2 and inhibits the Ras-Erk pathway downstream of protein-tyrosine kinases. Genes Cells. 2006.02; 11 (2): 143-151. ( PubMed, DOI )

Moriguchi T, Urushiyama S, Hisamoto N, Iemura S, Uchida S, Natsume T, Matsumoto K, Shibuya H. WNK1 regulates phosphorylation of cation-chloride-coupled cotransporters via the STE20-related kinases, SPAK and OSR1. J Biol Chem. 2005.12; 280 (52): 42685-42693. ( PubMed, DOI )

Ohnishi J, Ohnishi E, Shibuya H, Takahashi T. Functions for proteinases in the ovulatory process. Biochim Biophys Acta. 2005.08; 1751 (1): 95-109. ( PubMed, DOI )

Adachi S, Jigami T, Yasui T, Nakano T, Ohwada S, Omori Y, Sugano S, Ohkawara B, Shibuya H, Nakamura T, Akiyama T. Role of a BCL9-related beta-catenin-binding protein, B9L, in tumorigenesis induced by aberrant activation of Wnt signaling. Cancer Res. 2004.12; 64 (23): 8496-8501. ( PubMed, DOI )

Aoyama Y, Urushiyama S, Yamada M, Kato C, Ide H, Higuchi S, Akiyama T, Shibuya H. MFB-1, an F-box-type ubiquitin ligase, regulates TGF-beta signalling. Genes Cells. 2004.11; 9 (11): 1093-1101. ( PubMed, DOI )

Muraki M, Ohkawara B, Hosoya T, Onogi H, Koizumi J, Koizumi T, Sumi K, Yomoda J, Murray MV, Kimura H, Furuichi K, Shibuya H, Krainer AR, Suzuki M, Hagiwara M. Manipulation of alternative splicing by a newly developed inhibitor of Clks. J Biol Chem. 2004.06; 279 (23): 24246-24254. ( PubMed, DOI )

Ohkawara B, Shirakabe K, Hyodo-Miura J, Matsuo R, Ueno N, Matsumoto K, Shibuya H. Role of the TAK1-NLK-STAT3 pathway in TGF-beta-mediated mesoderm induction. Genes Dev. 2004.02; 18 (4): 381-386. ( PubMed, DOI )

Ishitani T, Takaesu G, Ninomiya-Tsuji J, Shibuya H, Gaynor RB, Matsumoto K. Role of the TAB2-related protein TAB3 in IL-1 and TNF signaling. EMBO J. 2003.12; 22 (23): 6277-6288. ( PubMed, DOI )

Yamada M, Ohkawara B, Ichimura N, Hyodo-Miura J, Urushiyama S, Shirakabe K, Shibuya H. Negative regulation of Wnt signalling by HMG2L1, a novel NLK-binding protein. Genes Cells. 2003.08; 8 (8): 677-684. ( PubMed )

Oishi I, Suzuki H, Onishi N, Takada R, Kani S, Ohkawara B, Koshida I, Suzuki K, Yamada G, Schwabe GC, Mundlos S, Shibuya H, Takada S, Minami Y. The receptor tyrosine kinase Ror2 is involved in non-canonical Wnt5a/JNK signalling pathway. Genes Cells. 2003.07; 8 (7): 645-654. ( PubMed )

Suzawa M, Takada I, Yanagisawa J, Ohtake F, Ogawa S, Yamauchi T, Kadowaki T, Takeuchi Y, Shibuya H, Gotoh Y, Matsumoto K, Kato S. Cytokines suppress adipogenesis and PPAR-gamma function through the TAK1/TAB1/NIK cascade. Nat Cell Biol. 2003.03; 5 (3): 224-230. ( PubMed, DOI )

Ishitani T, Kishida S, Hyodo-Miura J, Ueno N, Yasuda J, Waterman M, Shibuya H, Moon RT, Ninomiya-Tsuji J, Matsumoto K. The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling. Mol Cell Biol. 2003.01; 23 (1): 131-139. ( PubMed )

Komatsu Y, Shibuya H, Takeda N, Ninomiya-Tsuji J, Yasui T, Miyado K, Sekimoto T, Ueno N, Matsumoto K, Yamada G. Targeted disruption of the Tab1 gene causes embryonic lethality and defects in cardiovascular and lung morphogenesis. Mech Dev. 2002.12; 119 (2): 239-249. ( PubMed )

Hyodo-Miura J, Urushiyama S, Nagai S, Nishita M, Ueno N, Shibuya H. Involvement of NLK and Sox11 in neural induction in Xenopus development. Genes Cells. 2002.05; 7 (5): 487-496. ( PubMed )

Yanagisawa M, Nakashima K, Takeda K, Ochiai W, Takizawa T, Ueno M, Takizawa M, Shibuya H, Taga T. Inhibition of BMP2-induced, TAK1 kinase-mediated neurite outgrowth by Smad6 and Smad7. Genes Cells. 2001.12; 6 (12): 1091-1099. ( PubMed )

Shirakabe K, Terasawa K, Miyama K, Shibuya H, Nishida E. Regulation of the activity of the transcription factor Runx2 by two homeobox proteins, Msx2 and Dlx5. Genes Cells. 2001.10; 6 (10): 851-856. ( PubMed )

Sugawara K, Morita K, Ueno N, Shibuya H. BIP, a BRAM-interacting protein involved in TGF-beta signalling, regulates body length in Caenorhabditis elegans. Genes Cells. 2001.07; 6 (7): 599-606. ( PubMed )

Morita K, Shimizu M, Shibuya H, Ueno N. A DAF-1-binding protein BRA-1 is a negative regulator of DAF-7 TGF-beta signaling. Proc Natl Acad Sci U S A. 2001.05; 98 (11): 6284-6288. ( PubMed, DOI )

Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, Matsui H, Kimura A, Sawa H, Nakayama K, Shibuya H, Nagashima K, Takahashi T. Cloning and characterization of a rat ortholog of MMP-23 (matrix metalloproteinase-23), a unique type of membrane-anchored matrix metalloproteinase and conditioned switching of its expression during the ovarian follicular development. Mol Endocrinol. 2001.05; 15 (5): 747-764. ( PubMed )

Masuda Y, Sasaki A, Shibuya H, Ueno N, Ikeda K, Watanabe K. Dlxin-1, a novel protein that binds Dlx5 and regulates its transcriptional function. J Biol Chem. 2001.02; 276 (7): 5331-5338. ( PubMed, DOI )

Tago K, Nakamura T, Nishita M, Hyodo J, Nagai S, Murata Y, Adachi S, Ohwada S, Morishita Y, Shibuya H, Akiyama T. Inhibition of Wnt signaling by ICAT, a novel beta-catenin-interacting protein. Genes Dev. 2000.07; 14 (14): 1741-1749. ( PubMed )

Hanafusa H, Masuyama N, Kusakabe M, Shibuya H, Nishida E. The TGF-beta family member derriere is involved in regulation of the establishment of left-right asymmetry. EMBO Rep. 2000.07; 1 (1): 32-39. ( PubMed, DOI )

Kimura N, Matsuo R, Shibuya H, Nakashima K, Taga T. BMP2-induced apoptosis is mediated by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. J Biol Chem. 2000.06; 275 (23): 17647-17652. ( PubMed, DOI )

Takatsu Y, Nakamura M, Stapleton M, Danos MC, Matsumoto K, O'Connor MB, Shibuya H, Ueno N. TAK1 participates in c-Jun N-terminal kinase signaling during Drosophila development. Mol Cell Biol. 2000.05; 20 (9): 3015-3026. ( PubMed )

Takaesu G, Kishida S, Hiyama A, Yamaguchi K, Shibuya H, Irie K, Ninomiya-Tsuji J, Matsumoto K. TAB2, a novel adaptor protein, mediates activation of TAK1 MAPKKK by linking TAK1 to TRAF6 in the IL-1 signal transduction pathway. Mol Cell. 2000.04; 5 (4): 649-658. ( PubMed )

Nishita M, Hashimoto MK, Ogata S, Laurent MN, Ueno N, Shibuya H, Cho KW. Interaction between Wnt and TGF-beta signalling pathways during formation of Spemann's organizer. Nature. 2000.02; 403 (6771): 781-785. ( PubMed, DOI )

Nishita M, Ueno N, Shibuya H. Smad8B, a Smad8 splice variant lacking the SSXS site that inhibits Smad8-mediated signalling. Genes Cells. 1999.10; 4 (10): 583-591. ( PubMed )

Hanafusa H, Ninomiya-Tsuji J, Masuyama N, Nishita M, Fujisawa J, Shibuya H, Matsumoto K, Nishida E. Involvement of the p38 mitogen-activated protein kinase pathway in transforming growth factor-beta-induced gene expression. J Biol Chem. 1999.09; 274 (38): 27161-27167. ( PubMed )

Ishitani T, Ninomiya-Tsuji J, Nagai S, Nishita M, Meneghini M, Barker N, Waterman M, Bowerman B, Clevers H, Shibuya H, Matsumoto K. The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF. Nature. 1999.06; 399 (6738): 798-802. ( PubMed, DOI )

Masuyama N, Hanafusa H, Kusakabe M, Shibuya H, Nishida E. Identification of two Smad4 proteins in Xenopus. Their common and distinct properties. J Biol Chem. 1999.04; 274 (17): 12163-12170. ( PubMed )

Miyama K, Yamada G, Yamamoto TS, Takagi C, Miyado K, Sakai M, Ueno N, Shibuya H. A BMP-inducible gene, dlx5, regulates osteoblast differentiation and mesoderm induction. Dev Biol. 1999.04; 208 (1): 123-133. ( PubMed, DOI )

Hamada F, Tomoyasu Y, Takatsu Y, Nakamura M, Nagai S, Suzuki A, Fujita F, Shibuya H, Toyoshima K, Ueno N, Akiyama T. Negative regulation of Wingless signaling by D-axin, a Drosophila homolog of axin. Science. 1999.03; 283 (5408): 1739-1742. ( PubMed )

Yamaguchi K, Nagai S, Ninomiya-Tsuji J, Nishita M, Tamai K, Irie K, Ueno N, Nishida E, Shibuya H, Matsumoto K. XIAP, a cellular member of the inhibitor of apoptosis protein family, links the receptors to TAB1-TAK1 in the BMP signaling pathway. EMBO J. 1999.01; 18 (1): 179-187. ( PubMed, DOI )

Kurozumi K, Nishita M, Yamaguchi K, Fujita T, Ueno N, Shibuya H. BRAM1, a BMP receptor-associated molecule involved in BMP signalling. Genes Cells. 1998.04; 3 (4): 257-264. ( PubMed )

Shibuya H, Iwata H, Masuyama N, Gotoh Y, Yamaguchi K, Irie K, Matsumoto K, Nishida E, Ueno N. Role of TAK1 and TAB1 in BMP signaling in early Xenopus development. EMBO J. 1998.02; 17 (4): 1019-1028. ( PubMed, DOI )

Akiyama S, Katagiri T, Namiki M, Yamaji N, Yamamoto N, Miyama K, Shibuya H, Ueno N, Wozney JM, Suda T. Constitutively active BMP type I receptors transduce BMP-2 signals without the ligand in C2C12 myoblasts. Exp Cell Res. 1997.09; 235 (2): 362-369. ( PubMed, DOI )

Shirakabe K, Yamaguchi K, Shibuya H, Irie K, Matsuda S, Moriguchi T, Gotoh Y, Matsumoto K, Nishida E. TAK1 mediates the ceramide signaling to stress-activated protein kinase/c-Jun N-terminal kinase. J Biol Chem. 1997.03; 272 (13): 8141-8144. ( PubMed )

Moriguchi T, Kuroyanagi N, Yamaguchi K, Gotoh Y, Irie K, Kano T, Shirakabe K, Muro Y, Shibuya H, Matsumoto K, Nishida E, Hagiwara M. A novel kinase cascade mediated by mitogen-activated protein kinase kinase 6 and MKK3. J Biol Chem. 1996.06; 271 (23): 13675-13679. ( PubMed )

Shibuya H, Yamaguchi K, Shirakabe K, Tonegawa A, Gotoh Y, Ueno N, Irie K, Nishida E, Matsumoto K. TAB1: an activator of the TAK1 MAPKKK in TGF-beta signal transduction. Science. 1996.05; 272 (5265): 1179-1182. ( PubMed )

Yamaguchi K, Shirakabe K, Shibuya H, Irie K, Oishi I, Ueno N, Taniguchi T, Nishida E, Matsumoto K. Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction. Science. 1995.12; 270 (5244): 2008-2011. ( PubMed )

Shibuya H, Kohu K, Yamada K, Barsoumian EL, Perlmutter RM, Taniguchi T. Functional dissection of p56lck, a protein tyrosine kinase which mediates interleukin-2-induced activation of the c-fos gene. Mol Cell Biol. 1994.09; 14 (9): 5812-5819. ( PubMed )

Shibuya H, Yoneyama M, Ninomiya-Tsuji J, Matsumoto K, Taniguchi T. IL-2 and EGF receptors stimulate the hematopoietic cell cycle via different signaling pathways: demonstration of a novel role for c-myc. Cell. 1992.07; 70 (1): 57-67. ( PubMed )

Shibuya H, Irie K, Ninomiya-Tsuji J, Goebl M, Taniguchi T, Matsumoto K. New human gene encoding a positive modulator of HIV Tat-mediated transactivation. Nature. 1992.06; 357 (6380): 700-702. ( PubMed, DOI )

Hatakeyama M, Kawahara A, Mori H, Shibuya H, Taniguchi T. c-fos gene induction by interleukin 2: identification of the critical cytoplasmic regions within the interleukin 2 receptor beta chain. Proc Natl Acad Sci U S A. 1992.03; 89 (6): 2022-2026. ( PubMed )

Shibuya H, Yoneyama M, Nakamura Y, Harada H, Hatakeyama M, Minamoto S, Kono T, Doi T, White R, Taniguchi T. The human interleukin-2 receptor beta-chain gene: genomic organization, promoter analysis and chromosomal assignment. Nucleic Acids Res. 1990.07; 18 (13): 3697-3703. ( PubMed )

Shibuya H, Taniguchi T. Identification of multiple cis-elements and trans-acting factors involved in the induced expression of human IL-2 gene. Nucleic Acids Res. 1989.11; 17 (22): 9173-9184. ( PubMed )

Shibuya H, Yoneyama M, Taniguchi T. Involvement of a common transcription factor in the regulated expression of IL-2 and IL-2 receptor genes. Int Immunol. 1989; 1 (1): 43-49. ( PubMed )

Fujita T, Shibuya H, Hotta H, Yamanishi K, Taniguchi T. Interferon-beta gene regulation: tandemly repeated sequences of a synthetic 6 bp oligomer function as a virus-inducible enhancer. Cell. 1987.05; 49 (3): 357-367. ( PubMed )

Maruyama M, Shibuya H, Harada H, Hatakeyama M, Seiki M, Fujita T, Inoue J, Yoshida M, Taniguchi T. Evidence for aberrant activation of the interleukin-2 autocrine loop by HTLV-1-encoded p40x and T3/Ti complex triggering. Cell. 1987.01; 48 (2): 343-350. ( PubMed )

Fujita T, Shibuya H, Ohashi T, Yamanishi K, Taniguchi T. Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes. Cell. 1986.08; 46 (3): 401-405. ( PubMed )