基本情報

写真a

正木　慶昭(マサキ　ヨシアキ)

MASAKI YOSHIAKI


職名

准教授
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出身学校 【 表示 ／ 非表示 】

  • 早稲田大学  理工学部  応用化学科  2005年03月  卒業

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出身大学院 【 表示 ／ 非表示 】

  • 早稲田大学  理工学研究科  応用化学科  修士課程  2007年03月  修了

  • 東京工業大学  生命理工学研究科  分子生命科学専攻  博士課程  2010年03月  修了

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取得学位 【 表示 ／ 非表示 】

  • 理学博士  東京工業大学

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経歴(学内) 【 表示 ／ 非表示 】

  • 2023年09月
    -
    現在
    東京医科歯科大学 統合研究機構 先端医歯工学創成クラスター 核酸・ペプチド創薬治療研究センター 准教授
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経歴(学外) 【 表示 ／ 非表示 】

  • 米国スクリプス研究所 特任研究員
  • 東京工業大学 准教授
  • 東京工業大学 助教
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所属学協会 【 表示 ／ 非表示 】

  • 日本核酸化学会

  • 日本核酸医薬学会

  • 日本化学会

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研究分野 【 表示 ／ 非表示 】

  • 有機合成化学

  • 生物有機化学

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研究テーマ 【 表示 ／ 非表示 】

  • ひずみとゆるみによる核酸－タンパク質複合体形成の精密制御，2020年04月 - 2024年03月

  • 核酸－タンパク質複合体形成に最適化した毒性のない核酸医薬の開発，2017年04月 - 2021年03月

  • 変形能解析をもちいた新規糖部修飾核酸の合理的開発方法の確立，2014年04月 - 2017年03月

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論文・総説 【 表示 ／ 非表示 】

  1. Kanagawa T, Tachibana S, Masaki Y, Seio K. Synthesis and Conformational Analyses of Cyclonucleoside Having 13-Membered Ring Bridging Nucleobase and 5'-Position via a Linker Containing Sulfonamide. Organic letters. 2023.11; 25 (43): 7868-7872. ( PubMed, DOI )

  2. Kanagawa T, Koyoma A, Masaki Y, Seio K. Alteration of target cleavage patterns and off-target reduction of antisense oligonucleotides incorporating 2-N-carbamoyl- or (2-pyridyl)guanine. Organic & biomolecular chemistry. 2023.06; 21 (25): 5214-5224. ( PubMed, DOI )

  3. Masaki Y, Tabira A, Hattori S, Wakatsuki S, Seio K. Insertion of a methylene group into the backbone of an antisense oligonucleotide reveals the importance of deoxyribose recognition by RNase H. Organic & biomolecular chemistry. 2022.11; 20 (45): 8917-8924. ( PubMed, DOI )

  4. Tomori T, Uekusa K, Koyama A, Kanagawa T, Masaki Y, Seio K. Synthesis of 2'-O-[3-(N-methylsulfamoyl)propan-1-yl]ribothymidine as a potentially applicable 2'-modified nucleoside for antisense oligonucleotides. Bioorganic & medicinal chemistry. 2022.11; 73 117002. ( PubMed, DOI )

  5. Masaki Y, Onishi Y, Seio K. Quantification of synthetic errors during chemical synthesis of DNA and its suppression by non-canonical nucleosides. Scientific reports. 2022.07; 12 (1): 12095. ( PubMed, DOI )

  6. Okamura H, Trinh GH, Dong Z, Masaki Y, Seio K, Nagatsugi F. Selective and stable base pairing by alkynylated nucleosides featuring a spatially-separated recognition interface. Nucleic acids research. 2022.04; 50 (6): 3042-3055. ( PubMed, DOI )

  7. Masaki Y, Maruyama A, Yoshida K, Tomori T, Kishimura T, Seio K. Oligodeoxynucleotides Modified with 2'-O-(Cysteinylaminobutyl)carbamoylethylribothymidine Residues for Native Chemical Ligation with Peptide at Internal Positions. Bioconjugate chemistry. 2022.02; 33 (2): 272-278. ( PubMed, DOI )

  8. Kishimura T, Tomori T, Masaki Y, Seio K. Synthesis of 2'-O-alkylcarbamoylethyl-modified oligonucleotides with enhanced nuclease resistance that form isostable duplexes with complementary RNA. Bioorganic & medicinal chemistry letters. 2021.03; 35 127779. ( PubMed, DOI )

  9. Hara Y, Mizobe Y, Inoue YU, Hashimoto Y, Motohashi N, Masaki Y, Seio K, Takeda S, Nagata T, Wood MJA, Inoue T, Aoki Y. Novel EGFP reporter cell and mouse models for sensitive imaging and quantification of exon skipping. Scientific reports. 2020.06; 10 (1): 10110. ( PubMed, DOI )

  10. Takeshita L, Yamada Y, Masaki Y, Seio K. Synthesis of Deoxypseudouridine 5'-Triphosphate Bearing the Photoremovable Protecting Group at the N1 Position Capable of Enzymatic Incorporation to DNA. The Journal of organic chemistry. 2020.02; 85 (4): 1861-1870. ( PubMed, DOI )

  11. Seio K, Yamaguchi K, Yamazaki A, Kanamori T, Masaki Y. Transcription of DNA duplex containing deoxypseudouridine and deoxypseudoisocytidine, and inhibition of transcription by triplex forming oligonucleotide that recognizes the modified duplex. Nucleosides, nucleotides & nucleic acids. 2020; 39 (6): 892-904. ( PubMed, DOI )

  12. Masaki Y, Yamamoto K, Yoshida K, Maruyama A, Tomori T, Iriyama Y, Nakajima H, Kanaki T, Seio K. Modification of oligonucleotides with weak basic residues via the 2'-O-carbamoylethyl linker for improving nuclease resistance without loss of duplex stability and antisense activity. Organic & biomolecular chemistry. 2019.05; 17 (19): 4835-4842. ( PubMed, DOI )

  13. Kanamori T, Masaki Y, Oda Y, Ohzeki H, Ohkubo A, Sekine M, Seio K. DNA triplex-based fluorescence turn-on sensors for adenosine using a fluorescent molecular rotor 5-(3-methylbenzofuran-2-yl) deoxyuridine. Organic & biomolecular chemistry. 2019.02; 17 (8): 2077-2080. ( PubMed, DOI )

  14. Masaki Y, Inde T, Maruyama A, Seio K. Tolerance of N(2)-heteroaryl modifications on guanine bases in a DNA G-quadruplex. Organic & biomolecular chemistry. 2019.01; 17 (4): 859-866. ( PubMed, DOI )

  15. Masaki Y, Yamamoto K, Inde T, Yoshida K, Maruyama A, Nagata T, Tanihata J, Takeda S, Sekine M, Seio K. Synthesis of 2'-O-(N-methylcarbamoylethyl) 5-methyl-2-thiouridine and its application to splice-switching oligonucleotides. Bioorganic & medicinal chemistry letters. 2019.01; 29 (2): 160-163. ( PubMed, DOI )

  16. Masaki Y, Iriyama Y, Nakajima H, Kuroda Y, Kanaki T, Furukawa S, Sekine M, Seio K. Application of 2'-O-(2-N-Methylcarbamoylethyl) Nucleotides in RNase H-Dependent Antisense Oligonucleotides. Nucleic acid therapeutics. 2018.10; 28 (5): 307-311. ( PubMed, DOI )

  17. Tomori, T; Nagaoka, K; Takeshita, L; Shiozawa, T; Miyatake, Y; Masaki, Y; Sekine, M; Seio, K. Deoxynucleoside Triphosphate Containing Pyridazin-3-one Aglycon as a Thymidine Triphosphate Substitute for Primer Extension and Chain Elongation by Klenow Fragments JOURNAL OF ORGANIC CHEMISTRY. 2018.08; 83 (15): 8353-8363. ( PubMed, DOI )

  18. Seio, K; Kanamori, T; Masaki, Y. Solvent- and environment-dependent fluorescence of modified nucleobases TETRAHEDRON LETTERS. 2018.05; 59 (21): 1977-1985. ( DOI )

  19. Ohno K, Sugiyama D, Takeshita L, Kanamori T, Masaki Y, Sekine M, Seio K. Synthesis of photocaged 6-O-(2-nitrobenzyl)guanosine and 4-O-(2-nitrobenzyl) uridine triphosphates for photocontrol of the RNA transcription reaction. Bioorganic & medicinal chemistry. 2017.11; 25 (21): 6007-6015. ( PubMed, DOI )

  20. Inde T, Masaki Y, Maruyama A, Ito Y, Makio N, Miyatake Y, Tomori T, Sekine M, Seio K. Synthesis of oligonucleotides containing 2-N-heteroarylguanine residues and their effect on duplex/triplex stability. Organic & biomolecular chemistry. 2017.10; 15 (39): 8371-8383. ( PubMed, DOI )

  21. Masaki Y, Sekine M, Seio K. Deformability Calculation for Estimation of the Relative Stability of Chemically Modified RNA Duplexes. Current protocols in nucleic acid chemistry. 2017.03; 68 7.27.1-7.27.10. ( PubMed, DOI )

  22. Kanamori T, Takamura A, Tago N, Masaki Y, Ohkubo A, Sekine M, Seio K. Fluorescence enhancement of oligodeoxynucleotides modified with green fluorescent protein chromophore mimics upon triplex formation. Organic & biomolecular chemistry. 2017.02; 15 (5): 1190-1197. ( PubMed, DOI )

  23. Masaki Y, Ito H, Oda Y, Yamazaki K, Tago N, Ohno K, Ishii N, Tsunoda H, Kanamori T, Ohkubo A, Sekine M, Seio K. Enzymatic synthesis and reverse transcription of RNAs incorporating 2'-O-carbamoyl uridine triphosphate. Chemical communications (Cambridge, England). 2016.10; 52 (87): 12889-12892. ( PubMed, DOI )

  24. Seio K, Ohno Y, Ohno K, Takeshita L, Kanamori T, Masaki Y, Sekine M. Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl]thymidine. Bioorganic & medicinal chemistry letters. 2016.10; 26 (19): 4861-4863. ( PubMed, DOI )

  25. Asami H, Tokugawa M, Masaki Y, Ishiuchi S, Gloaguen E, Seio K, Saigusa H, Fujii M, Sekine M, Mons M. Effective Strategy for Conformer-Selective Detection of Short-Lived Excited State Species: Application to the IR Spectroscopy of the N1H Keto Tautomer of Guanine. The journal of physical chemistry. A. 2016.04; 120 (14): 2179-84. ( PubMed, DOI )

  26. Tokugawa M, Masaki Y, Canggadibrata JC, Kaneko K, Shiozawa T, Kanamori T, Grøtli M, Wilhelmsson LM, Sekine M, Seio K. 7-(Benzofuran-2-yl)-7-deazadeoxyguanosine as a fluorescence turn-ON probe for single-strand DNA binding protein. Chemical communications (Cambridge, England). 2016.03; 52 (19): 3809-12. ( PubMed, DOI )

  27. Ito Y, Masaki Y, Kanamori T, Ohkubo A, Seio K, Sekine M. Synthesis of 5-[3-(2-aminopyrimidin-4-yl)aminopropyn-1-yl]uracil derivative that recognizes Ade-Thy base pairs in double-stranded DNA. Bioorganic & medicinal chemistry letters. 2016.01; 26 (1): 194-6. ( PubMed, DOI )

  28. Ohkubo A, Yamada K, Ito Y, Yoshimura K, Miyauchi K, Kanamori T, Masaki Y, Seio K, Yuasa H, Sekine M. Synthesis and triplex-forming properties of oligonucleotides capable of recognizing corresponding DNA duplexes containing four base pairs. Nucleic acids research. 2015.07; 43 (12): 5675-86. ( PubMed, DOI )

  29. Tomori T, Miyatake Y, Sato Y, Kanamori T, Masaki Y, Ohkubo A, Sekine M, Seio K. Synthesis of peptide nucleic acids containing pyridazine derivatives as cytosine and thymine analogs, and their duplexes with complementary oligodeoxynucleotides. Organic letters. 2015.03; 17 (6): 1609-12. ( PubMed, DOI )

  30. Kanamori T, Ohzeki H, Masaki Y, Ohkubo A, Takahashi M, Tsuda K, Ito T, Shirouzu M, Kuwasako K, Muto Y, Sekine M, Seio K. Controlling the fluorescence of benzofuran-modified uracil residues in oligonucleotides by triple-helix formation. Chembiochem : a European journal of chemical biology. 2015.01; 16 (1): 167-76. ( PubMed, DOI )

  31. Yamada T, Masaki Y, Okaniwa N, Kanamori T, Ohkubo A, Tsunoda H, Seio K, Sekine M. Synthesis and properties of oligonucleotides modified with 2'-O-(2-carboxyethyl)nucleotides and their carbamoyl derivatives. Organic & biomolecular chemistry. 2014.09; 12 (33): 6457-64. ( PubMed, DOI )

  32. Yamada K, Masaki Y, Tsunoda H, Ohkubo A, Seio K, Sekine M. A new modified cytosine base capable of base pairing with guanine using four hydrogen bonds. Organic & biomolecular chemistry. 2014.04; 12 (14): 2255-62. ( PubMed, DOI )

  33. Masaki Y, Miyasaka R, Hirai K, Kanamori T, Tsunoda H, Ohkubo A, Seio K, Sekine M. Properties of 5- and/or 2-modified 2'-O-cyanoethyl uridine residue: 2'-O-cyanoethyl-5-propynyl-2-thiouridine as an efficient duplex stabilizing component. Organic & biomolecular chemistry. 2014.02; 12 (7): 1157-62. ( PubMed, DOI )

  34. Iijima Y, Kojima S, Kodama E, Kurohagi S, Kanamori T, Masaki Y, Ohkubo A, Sekine M, Seio K. Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3'-terminus. Organic & biomolecular chemistry. 2013.12; 11 (47): 8276-82. ( PubMed, DOI )

  35. Ohkubo A, Kondo Y, Suzuki M, Kobayashi H, Kanamori T, Masaki Y, Seio K, Nagai K, Sekine M. Chemical synthesis of U1 snRNA derivatives. Organic letters. 2013.09; 15 (17): 4386-9. ( PubMed, DOI )

  36. Yamada K, Ohkubo A, Esaka Y, Kanamori T, Masaki Y, Seio K, Sekine M. Base recognition of gap sites in DNA-DNA and DNA-RNA duplexes by short oligonucleotides. Bioorganic & medicinal chemistry letters. 2013.06; 23 (11): 3448-51. ( PubMed, DOI )

  37. Seio K, Kanamori T, Tokugawa M, Ohzeki H, Masaki Y, Tsunoda H, Ohkubo A, Sekine M. Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine. Bioorganic & medicinal chemistry. 2013.06; 21 (11): 3197-201. ( PubMed, DOI )

  38. Masaki Y, Miyasaka R, Hirai K, Tsunoda H, Ohkubo A, Seio K, Sekine M. Prediction of the stability of modified RNA duplexes based on deformability analysis: oligoribonucleotide derivatives modified with 2'-O-cyanoethyl-5-propynyl-2-thiouridine as a promising component. Chemical communications (Cambridge, England). 2012.07; 48 (58): 7313-5. ( PubMed, DOI )

  39. Seio K, Kurohagi S, Kodama E, Masaki Y, Tsunoda H, Ohkubo A, Sekine M. Short-RNA selective binding of oligonucleotides modified using adenosine and guanosine derivatives that possess cyclohexyl phosphates as substituents. Organic & biomolecular chemistry. 2012.02; 10 (5): 994-1006. ( PubMed, DOI )

  40. Tsunoda H, Kudo T, Masaki Y, Ohkubo A, Seio K, Sekine M. Biochemical behavior of N-oxidized cytosine and adenine bases in DNA polymerase-mediated primer extension reactions. Nucleic acids research. 2011.04; 39 (7): 2995-3004. ( PubMed, DOI )

  41. Masaki Y, Miyasaka R, Ohkubo A, Seio K, Sekine M. Linear relationship between deformability and thermal stability of 2'-O-modified RNA hetero duplexes. The journal of physical chemistry. B. 2010.02; 114 (7): 2517-24. ( PubMed, DOI )

  42. Masaki Y, Ohkubo A, Seio K, Sekine M. Synthesis of 6-N-(benzothiazol-2-yl)deoxyadenosine and its exciton-coupled circular dichroism. Bioorganic & medicinal chemistry. 2010.01; 18 (2): 567-72. ( PubMed, DOI )

  43. Seio K, Takaku Y, Miyazaki K, Kurohagi S, Masaki Y, Ohkubo A, Sekine M. Synthesis of terminally modified oligonucleotides and their hybridization dependence on the size of the target RNAs. Organic & biomolecular chemistry. 2009.06; 7 (11): 2440-51. ( PubMed, DOI )

  44. Seio K, Miyazaki K, Kurohagi S, Masaki Y, Tsunoda H, Ohkubo A, Sekine M. Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization. Nucleic acids symposium series (2004). 2009; (53): 13-4. ( PubMed, DOI )

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講演・口頭発表等 【 表示 ／ 非表示 】

  1. 島村大輝, 有江嶺美, 金山朱里, 宮内彩, 清尾康志, 正木慶昭. 細胞選択的送達を目指した糖リガンド修飾アンチセンス核酸の合成と性質. 日本化学会第104春季年会 2024.03

  2. 立花茂載, 大西里絵, 正木慶昭, 清尾康志. 5´位を増炭したスルホンアミド骨格を有するチミジンダイマーの合成と物性評価. 日本化学会第104春季年会 2024.03

  3. 瀧川駿太朗, 友利貴人, 植草宏哉, 正木慶昭, 清尾康志. 2´水酸基にスルホンアミド修飾またはスルホネート修飾を有する5-メチルウリジン誘導体の合成および二重鎖形成能の評価. 日本化学会第104春季年会 2024.03

  4. 田平彩乃, 若月駿介, 清尾 康志, 正木 慶昭. 置換基を導入した2´-O, 3´-C架橋アラビノフラノシルチミンを含むオリゴヌクレオチドの合成と性質. 日本化学会第104春季年会 2024.03

  5. 鈴木雄大, 須田雪音, 井分彩乃, 正木慶昭, 清尾 康志. 光架橋ビオチンリンカーを有するグアノシンテトラリン酸類似体の合成. 日本化学会第104春季年会 2024.03

  6. 宮地健人, 羽生愛深, 清尾康志, 正木慶昭. 7位にピリジル基を導入した7-デアザデオキシグアノシンを含むオリゴヌクレオチドの合成と性質. 日本化学会第104春季年会 2024.03

  7. Masaki Y, Onishi Y, Seio K. Estimation of synthetic errors toward long DNA chemical synthesis. FIBER International Summit for Nucleic Acids (S-FISNA) 2024.02

  8. Tabira A, Httori S, Seio K, Masaki Y. Extended phosphate backbone of ASO reveals importance of deoxyribose recognition by RNase H. FIBER International Summit for Nucleic Acids (S-FISNA) 2024.02

  9. 清尾康志, 大西里絵, 立花茂載, 正木慶昭. スルホンアミド型バックボーンを導入したギャップマーの合成およびアンチセンス核酸としての性質. 日本核酸医薬学会 第8回年会 2023.07

  10. 田平彩乃, 服部詩萌里, 清尾康志, 正木慶昭. メチレン基のリン酸バックボーンへの挿入がRNase Hによるミスマッチを含む二重差切断に与える影響. 日本核酸医薬学会 第8回年会 2023.07

  11. 金川峻幸, 小山彩, 正木慶昭, 清尾康志. 塩基部修飾グアニン導入によるアンチセンス核酸の標的切断変化とオフターゲット抑制. 日本核酸医薬学会 第7回年会 2022.07

  12. 正木慶昭, 田平彩乃, 金川峻幸, 若月駿介, 服部詩萌里, 小山彩, 井上敦, 清尾康志. RNase H依存型アンチセンス核酸の安全性向上に向けた化学修飾の開発. 日本核酸医薬学会 第7回年会 2022.07

  13. 金川峻幸, 立花茂載, 大西里絵, 正木慶昭, 清尾康志. スルホンアミド構造を有する環状チミジン誘導体の合成とその構造解析. 日本核酸医薬学会 第7回年会 2022.07

  14. 植草宏哉, 友利貴人, 正木慶昭, 清尾康志. 2’-O-スルファモイルアルキル修飾を導入したオリゴヌクレオチドの合成と性質. 日本核酸医薬学会 第7回年会 2022.07

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受賞学術賞 【 表示 ／ 非表示 】

  • 奨励賞，日本核酸医薬学会，2022年08月

  • 東工大挑戦的研究賞　末松特別賞受賞，東京工業大学，2019年09月

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