Biomedical engineering

Elucidation of blood (liquid) flow effect on human renal development by renal organoid with perfusion culture system, 2017.04 - 2020.03

Summary： The purpose of this study is establishment in vitro perfusion culture system and to elucidate the effects for renal organoids (developmental stage) originated from human iPS cells on perfusion stimulation. With the air-liquid interface perfusion culture system, renal organoid cultured at an adequate perfusion rate was caused liquid diffusion and improved the organization by epithelial cells, while decayed it at a perfusion range above the adequate value. It was suggested that high perfusion rate caused a decrease in signal transduction for renal development in renal organoids.

inhibition effect of renal fibrosis with administration topically sustained HGF by HGF-expressing cell sheet transplantation, 2015.04 - 2017.03

Summary： Before examination of antifibrotic effect by HGF expressing cell-sheets transplantation, we analyzed the HGF protein distribution after administration of HGF containing solution on the kidney surface. Infiltrating-HGF via the interstitium or capillary vessels was observed inner renal tissue.Inner hHGF-positive cells was also observed in rat ischemic reperfusion injury kidney model 24 hours after HGF expressing cell-sheets transplantation. Although the treatment of transplanted HGF expressing cell-sheet showed unremarkable improving renal function and tissue disorder in rat cyclosporin nephropathy, tubular atrophy and myofibroblasts decreased. In this research, it was suggested that sustained inhibitory effect for fibrosis with HGF-expressing cell-sheet transplantation. However, there was also the effect of HGF on early renal disorder correlated to chronic fibrosis. Therefore, we have to verify an availability for antifibrotic effect of sustained HGF administration with cell-sheet.