Nagoya University, Graduate School, Division of Engineering, Graduated

Nagoya University, Graduated

Japan Society of Computer Aided Surgery

The Robotics Society of Japan

The Japanese Society for Regenerative Medicine

The Japan Society of Mechanical Engineers

Japanese Society for Medical and Biological Engineering

Cell biology

Biomedical engineering

Mechanics and mechatronics

Medical systems

Mechanics of materials and materials

Development of the differentiation derivative methods from induced tissue-specific pancreatic stem cells using TASCL to insulin excretory cells, 2019.04 - 2022.03

Summary： In this study, we succeeded in producing the cell mass of artificial insulin excretory cells from induced tissue specific pancreatic stem (iTS-P) cells using microdevice TASC. We were posssible to make the cell mass which is necessary for transplantation in large quantities concurrently. Furthermore, we transplanted the insulin excretory cells under the capsule of the kidney of diabetes mouse and confirmed a diachronic descent effect of the blood glucose level.

The Fate of Frozen Cells: Development of Quality Assessment Technology for Therapeutic Cells, 2018.04 - 2021.03

Summary： The purpose of this research is to "clarify the fate and intracellular potential of frozen cells" and "construct quality evaluation technology for therapeutic cells". Specifically, we conducted "cellular characteristics," "safety," "comprehensive analysis by metabolomics and epigenetics," and "quality judgment and prediction from cell and tissue images" of thawed cells and tissues, and were able to construct an effective quality evaluation technology for use in the fields of regenerative medicine, transplantation medicine, and assisted reproduction. In addition, hierarchical cluster analysis by metabolomics allows us to propose new candidate molecules (evaluation indicators) for quality assurance of frozen cells. By combining various evaluation methods in addition to conventional cell characteristics, it is possible to obtain more accurate information about the state of cells and tissues.

The visualization of microtissues using near-infrared fluorescence endoscope, 2016.04 - 2019.03

Summary： Near-infrared fluorescence endoscope system with indocyanine green (ICG) is a powerful tool to observe the interior tissues and organs in surgery in real time. It is useful especially in a wide range of surgical fields. Since ICG has strong near-infrared fluorescence, it can be visualized while excluding the effect of autofluorescence of tissues. In this study, we successfully labeled microtissues with ICG and then visualized them in real time. The near-infrared fluorescence endoscope system was helpful to observe microtissues with different sizes at high sensitivity (about 100-10,000 cell clusters) in vitro (the surface of pig small intestine, etc). Although the noise was amplified with increasing the sensitivity, it can be reduced by adjusting the threshold level. Our system can contribute to improving the safety of transplantation in regenerative therapy and cell medicine, and its medical applications will be expanded more and more in the future.

Development of cryopreservation system for cells and tissue constructs in regenerative medicine and transplantation, 2015.04 - 2018.03

Summary： In this study, we developed a novel (1) DMSO-free cryopreservation solution, (2) culture device for cryopreservation, and (3) freezing system. (1) In order to safely freeze cells, it is necessary to solve the problems such as the cytotoxicity of DMSO, the antigenicity change of animal-derived materials and the possibility of contamination with pathogens. We found an optimal composition of cryopreservation solution without DMSO and serum for human cells. (2) 3D culture device TASCL was used to create a large amount of human tissue-derived stem cell constructs having a uniform size. In addition, we successfully determined a vitrified freezing condition based on the effective composition of the freezing solution. (3) In order to control cell freezing, we succeeded in developing a freezing system with a stirling engine and in optimizing freezing conditions. Consequently, a cryopreservation system of cell tissue construct for regeneration and transplantation medical care was established.