Li F, Ichinose K, Ishibashi S, Yamamoto S, Iwasawa E, Suzuki M, Yoshida-Tanaka K, Yoshioka K, Nagata T, Hirabayashi H, Mogushi K, Yokota T. Preferential delivery of lipid-ligand conjugated DNA/RNA heteroduplex oligonucleotide to ischemic brain in hyperacute stage. Molecular therapy : the journal of the American Society of Gene Therapy. 2023.04; 31 (4): 1106-1122. ( PubMed, DOI )

Jia C, Lei Mon SS, Yang Y, Katsuyama M, Yoshida-Tanaka K, Nagata T, Yoshioka K, Yokota T. Change of intracellular calcium level causes acute neurotoxicity by antisense oligonucleotides via CSF route. Molecular therapy. Nucleic acids. 2023.03; 31 182-196. ( PubMed, DOI )

Amano A, Sanjo N, Araki W, Anraku Y, Nakakido M, Matsubara E, Tomiyama T, Nagata T, Tsumoto K, Kataoka K, Yokota T. Peripheral administration of nanomicelle-encapsulated anti-Aβ oligomer fragment antibody reduces various toxic Aβ species in the brain. Journal of nanobiotechnology. 2023.01; 21 (1): 36. ( PubMed, DOI )

Goto A, Yamamoto S, Igari T, Matsumoto SI, Chisaki I, Iida K, Nakayama M, Oda A, Kakoi Y, Uchida A, Miyata K, Nishikawa M, Nagata T, Kusuhara H, Yokota T, Hirabayashi H. Quantitative Model Analysis and Simulation of Pharmacokinetics and Metastasis-Associated Lung Adenocarcinoma 1 RNA Knockdown Effect After Systemic Administration of Cholesterol-Conjugated DNA/RNA Heteroduplex Oligonucleotide Crossing Blood-Brain Barrier of Mice. The Journal of pharmacology and experimental therapeutics. 2023.01; 384 (1): 197-204. ( PubMed, DOI )

Nishi R, Ohyagi M, Nagata T, Mabuchi Y, Yokota T. Regulation of activated microglia and macrophages by systemically administered DNA/RNA heteroduplex oligonucleotides. Molecular therapy : the journal of the American Society of Gene Therapy. 2022.06; 30 (6): 2210-2223. ( PubMed, DOI )

Miyashita A, Kobayashi M, Ishibashi S, Nagata T, Chandrasekhar A, Zochodne DW, Yokota T. The Role of Long Noncoding RNA MALAT1 in Diabetic Polyneuropathy and the Impact of Its Silencing in the Dorsal Root Ganglion by a DNA/RNA Heteroduplex Oligonucleotide. Diabetes. 2022.06; 71 (6): 1299-1312. ( PubMed, DOI )

Kaburagi H, Nagata T, Enomoto M, Hirai T, Ohyagi M, Ihara K, Yoshida-Tanaka K, Ebihara S, Asada K, Yokoyama H, Okawa A, Yokota T. Systemic DNA/RNA heteroduplex oligonucleotide administration for regulating the gene expression of dorsal root ganglion and sciatic nerve. Molecular therapy. Nucleic acids. 2022.06; 28 910-919. ( PubMed, DOI )

Nagata T, Dwyer CA, Yoshida-Tanaka K, Ihara K, Ohyagi M, Kaburagi H, Miyata H, Ebihara S, Yoshioka K, Ishii T, Miyata K, Miyata K, Powers B, Igari T, Yamamoto S, Arimura N, Hirabayashi H, Uchihara T, Hara RI, Wada T, Bennett CF, Seth PP, Rigo F, Yokota T. Cholesterol-functionalized DNA/RNA heteroduplexes cross the blood-brain barrier and knock down genes in the rodent CNS. Nature biotechnology. 2021.12; 39 (12): 1529-1536. ( PubMed, DOI )

Ohyagi M, Nagata T, Ihara K, Yoshida-Tanaka K, Nishi R, Miyata H, Abe A, Mabuchi Y, Akazawa C, Yokota T. DNA/RNA heteroduplex oligonucleotide technology for regulating lymphocytes in vivo. Nature communications. 2021.12; 12 (1): 7344. ( PubMed, DOI )

Motohiro Suzuki, Satoru Ishibashi, Eri Iwasawa , Takahiro Oguma, Yasuhiro Saito, Fuying Li, Shinichi Otsu, Keiko Ichinose, Kotaro Yoshioka, Tetsuya Nagata, Takanori Yokota. Effective silencing of miR-126 after ischemic stroke by means of intravenous α-tocopherol-conjugated heteroduplex oligonucleotide in mice. Scientific reports. 2021.07; 11 (1): 14237. ( PubMed, DOI )

Asada K, Sakaue F, Nagata T, Zhang JC, Yoshida-Tanaka K, Abe A, Nawa M, Nishina K, Yokota T. Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. Nucleic acids research. 2021.05; 49 (9): 4864-4876. ( PubMed, DOI )

Ono D, Asada K, Yui D, Sakaue F, Yoshioka K, Nagata T, Yokota T. Separation-related rapid nuclear transport of DNA/RNA heteroduplex oligonucleotide: unveiling distinctive intracellular trafficking. Molecular therapy. Nucleic acids. 2021.03; 23 1360-1370. ( PubMed, DOI )

Yuki Kishimoto, Osamu Nakagawa, Akane Fujii, Kotaro Yoshioka, Tetsuya Nagata, Takanori Yokota, Yoshiyuki Hari, Satoshi Obika. 2',4'-BNA/LNA with 9-(2-Aminoethoxy)-1,3-diaza-2-oxophenoxazine Efficiently Forms Duplexes and Has Enhanced Enzymatic Resistance*. Chemistry (Weinheim an der Bergstrasse, Germany). 2021.02; 27 (7): 2427-2438. ( PubMed, DOI )

Yutaro Asami, Tetsuya Nagata, Kotaro Yoshioka, Taiki Kunieda, Kie Yoshida-Tanaka, C Frank Bennett, Punit P Seth, Takanori Yokota. Efficient Gene Suppression by DNA/DNA Double-Stranded Oligonucleotide In Vivo. Molecular therapy : the journal of the American Society of Gene Therapy. 2021.02; 29 (2): 838-847. ( PubMed, DOI )

Naito M, Chaya H, Toh K, Kim BS, Hayashi K, Fukushima S, Nagata T, Yokota T, Kataoka K, Miyata K. Structural tuning of oligonucleotides for enhanced blood circulation properties of unit polyion complexes prepared from two-branched poly(ethylene glycol)-block-poly(l-lysine). Journal of controlled release : official journal of the Controlled Release Society. 2021.02; 330 812-820. ( PubMed, DOI )

Nagata Tetsuya. BBB透過性ヘテロ二重鎖オリゴヌクレオチド(BBB-penetrating heteroduplex oligonucleotide) 臨床神経学. 2020.11; 60 (Suppl.): S255. ( ichushi )

Ohyagi Masaki, Nagata Tetsuya, Ihara Kensuke, Nishi Rieko, Mabuchi Yo, Akazawa Chihiro, Yokota Takanori. DNA/RNAヘテロ二重鎖オリゴヌクレオチド技術を用いた多発性硬化症に対する新規治療戦略(New therapeutic strategy for multiple sclerosis by DNA/RNA heteroduplex oligonucleotide technology) 臨床神経学. 2020.11; 60 (Suppl.): S293. ( ichushi )

Jia Chunyan, Yoshioka Kotaro, Mon Su Su Lei, Tanaka Kie, Nagata Tetsuya, Yokota Takanori. CNS疾患に対するヘテロ二重鎖オリゴヌクレオチド使用による治療指標の改善(Hetereoduplex oligonucleotides technology improves therapeutic index for CNS diseases) 臨床神経学. 2020.11; 60 (Suppl.): S323. ( ichushi )

Yokota Takanori, Nagata Tetsuya, Dwyer Chrissa, Yoshida Kie, Yoshioka Kotaro, Seth Punit, Rigo Frank, Bennett Frank. BBB透過性ヘテロ二重鎖オリゴヌクレオチド(HDO)の開発(Development of BBB-penetrating heteroduplex oligonucleotides(HDO)) 臨床神経学. 2020.11; 60 (Suppl.): S314. ( ichushi )

Mishra V, Re DB, Le Verche V, Alvarez MJ, Vasciaveo A, Jacquier A, Doulias PT, Greco TM, Nizzardo M, Papadimitriou D, Nagata T, Rinchetti P, Perez-Torres EJ, Politi KA, Ikiz B, Clare K, Than ME, Corti S, Ischiropoulos H, Lotti F, Califano A, Przedborski S. Systematic elucidation of neuron-astrocyte interaction in models of amyotrophic lateral sclerosis using multi-modal integrated bioinformatics workflow. Nature communications. 2020.11; 11 (1): 5579. ( PubMed, DOI )

Kim BS, Naito M, Chaya H, Hori M, Hayashi K, Min HS, Yi Y, Kim HJ, Nagata T, Anraku Y, Kishimura A, Kataoka K, Miyata K. Noncovalent Stabilization of Vesicular Polyion Complexes with Chemically Modified/Single-Stranded Oligonucleotides and PEG-b-guanidinylated Polypeptides for Intracavity Encapsulation of Effector Enzymes Aimed at Cooperative Gene Knockdown. Biomacromolecules. 2020.10; 21 (10): 4365-4376. ( PubMed, DOI )

Teramoto N, Sugihara H, Yamanouchi K, Nakamura K, Kimura K, Okano T, Shiga T, Shirakawa T, Matsuo M, Nagata T, Daimon M, Matsuwaki T, Nishihara M. Pathological evaluation of rats carrying in-frame mutations in the dystrophin gene: a new model of Becker muscular dystrophy. Disease models & mechanisms. 2020.09; 13 (9): ( PubMed, DOI )

Hara Y, Mizobe Y, Inoue YU, Hashimoto Y, Motohashi N, Masaki Y, Seio K, Takeda S, Nagata T, Wood MJA, Inoue T, Aoki Y. Novel EGFP reporter cell and mouse models for sensitive imaging and quantification of exon skipping. Scientific reports. 2020.06; 10 (1): 10110. ( PubMed, DOI )

Lei Mon SS, Yoshioka K, Jia C, Kunieda T, Asami Y, Yoshida-Tanaka K, Piao W, Kuwahara H, Nishina K, Nagata T, Yokota T. Highly efficient gene silencing in mouse brain by overhanging-duplex oligonucleotides via intraventricular route. FEBS letters. 2020.05; 594 (9): 1413-1423. ( PubMed, DOI )

Yokoyama H, Hirai T, Nagata T, Enomoto M, Kaburagi H, Leiyo L, Motoyoshi T, Yoshii T, Okawa A, Yokota T. DNA Microarray Analysis of Differential Gene Expression in the Dorsal Root Ganglia of Four Different Neuropathic Pain Mouse Models. Journal of pain research. 2020; 13 3031-3043. ( PubMed, DOI )

Yoshioka Kotaro, Su Su Lei Mon , Jia Chunyan, Yamada Hiroki, Asami Yutaro, Tanaka Kie, Wenying Piao, Kuwahara Hiroya, Nishina Kazutaka, Nagata Tetsuya, Yokota Takanori. CNSを標的としたオーバーハングヘテロ二2本鎖核酸を用いた新しいアンチセンスオリゴヌクレオチド治療(A novel antisense oligonucleotide therapy by overhanging heteroduplex oligonucleotide targeting CNS) 臨床神経学. 2019.11; 59 (Suppl.): S408. ( ichushi )

Yoshioka K, Kunieda T, Asami Y, Guo H, Miyata H, Yoshida-Tanaka K, Sujino Y, Piao W, Kuwahara H, Nishina K, Hara RI, Nagata T, Wada T, Obika S, Yokota T. Highly efficient silencing of microRNA by heteroduplex oligonucleotides. Nucleic acids research. 2019.08; 47 (14): 7321-7332. ( PubMed, DOI )

Uehara T, Choong CJ, Nakamori M, Hayakawa H, Nishiyama K, Kasahara Y, Baba K, Nagata T, Yokota T, Tsuda H, Obika S, Mochizuki H. Amido-bridged nucleic acid (AmNA)-modified antisense oligonucleotides targeting α-synuclein as a novel therapy for Parkinson's disease. Scientific reports. 2019.05; 9 (1): 7567. ( PubMed, DOI )

Yoshiaki Masaki, Keishi Yamamoto, Takeshi Inde, Keita Yoshida, Atsuya Maruyama, Tetsuya Nagata, Jun Tanihata, Shin'ichi Takeda, Mitsuo Sekine, Kohji Seio. Synthesis of 2'-O-(N-methylcarbamoylethyl) 5-methyl-2-thiouridine and its application to splice-switching oligonucleotides. Bioorg. Med. Chem. Lett.. 2018.12; 29 (2): 160-163. ( PubMed, DOI )

Nagata Tetsuya. DNA/RNAヘテロ2本鎖核酸の最近の進歩(Recent progress of DNA/RNA heteroduplex oligonucleotide) 臨床神経学. 2018.12; 58 (Suppl.): S47. ( ichushi )

Kenji Rowel Q Lim*, Yusuke Echigoya*, Tetsuya Nagata*, Mutsuki Kuraoka, Masanori Kobayashi, Yoshitsugu Aoki, Terence Partridge, Rika Maruyama, Shin'ichi Takeda, Toshifumi Yokota *Equal Contribution . Efficacy of Multi-exon Skipping Treatment in Duchenne Muscular Dystrophy Dog Model Neonates. Mol. Ther.. 2018.10; ( PubMed, DOI )

Naoki Watanabe*, Tetsuya Nagata*, Youhei Satou, Satoru Masuda, Takashi Saito, Hidetoshi Kitagawa, Hirofumi Komaki, Kazuchika Takagaki, Shin'ichi Takeda *Equal Contribution. NS-065/NCNP-01: An Antisense Oligonucleotide for Potential Treatment of Exon 53 Skipping in Duchenne Muscular Dystrophy. Mol Ther Nucleic Acids. 2018.09; 13 442-449. ( PubMed, DOI )

Hara Y, Mizobe Y, Miyatake S, Takizawa H, Nagata T, Yokota T, Takeda S, Aoki Y. Exon Skipping Using Antisense Oligonucleotides for Laminin-Alpha2-Deficient Muscular Dystrophy. Methods in molecular biology (Clifton, N.J.). 2018; 1828 553-564. ( PubMed, DOI )

Uehara T., Choong C. J., Hayakawa H., Kasahara Y., Nagata T., Yokota T., Baba K., Nakamori M., Obika S., Mochizuki H.. Antisense oligonucleotides containing amido-bridged nucleic acid reduce SNCA expression and improve motor function in Parkinson's disease animal models JOURNAL OF THE NEUROLOGICAL SCIENCES. 2017.10; 381 1044-1045. ( DOI )

Yoshioka K., Kunieda T., Asami Y., Sujino Y., Tanaka K., Piao W., Kuwahara H., Nishina K., Nagata T., Yokota T.. Dual overhanging-duplex oligonucleotide improved efficacy and safety in gene therapy for FAP JOURNAL OF THE NEUROLOGICAL SCIENCES. 2017.10; 381 140. ( DOI )

Hitoshi Suzuki, Yoshitsugu Aoki, Toshiki Kameyama, Takashi Saito, Satoru Masuda, Jun Tanihata, Tetsuya Nagata, Akila Mayeda, Shin'ichi Takeda, Toshifumi Tsukahara. Endogenous Multiple Exon Skipping and Back-Splicing at the DMD Mutation Hotspot. Int J Mol Sci. 2016.10; 17 (10): ( PubMed, DOI )

Koichi Kimura, Hiroyuki Morita, Masao Daimon, Masaru Horio, Takayuki Kawata, Tomoko Nakao, Megumi Hirokawa, Ruriko Kitao, Daisuke Watanabe, Tetsuo Komori, Tetsuya Nagata, Shin'ichi Takeda, Hirofumi Komaki, Kazuhiko Segawa, Takashi Nakajima, Katsu Takenaka, Issei Komuro. Utility of Cystatin C for Estimating Glomerular Filtration Rate in Patients With Muscular Dystrophy. Int Heart J. 2016.05; 57 (3): 386-388. ( PubMed, DOI )

Mutsuki Kuraoka, En Kimura, Tetsuya Nagata, Takashi Okada, Yoshitsugu Aoki, Hisateru Tachimori, Naohiro Yonemoto, Michihiro Imamura, Shin'ichi Takeda. Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Am. J. Pathol.. 2016.05; 186 (5): 1302-1312. ( PubMed, DOI )

Masaki Y, Inde T, Nagata T, Tanihata J, Kanamori T, Seio K, Takeda S, Sekine M. Enhancement of exon skipping in mdx52 mice by 2'-O-methyl-2-thioribothymidine incorporation into phosphorothioate oligonucleotides Med Chem Commun. 2015.04; 6 630-633. ( DOI )